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Literature summary for 1.1.1.244 extracted from

  • Kloosterman, H.; Vrijbloed, J.W.; Dijkhuizen, L.
    Molecular, biochemical, and functional characterization of a Nudix hydrolase protein that stimulates the activity of a nicotinoprotein alcohol dehydrogenase (2002), J. Biol. Chem., 277, 34785-34792.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information strongly stimulated by the ACT protein Bacillus methanolicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, full activity only when grown in Mg2+ containing medium Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NAD+ native enzyme, pH 9.5, 50┬░C Bacillus methanolicus
0.04
-
NAD+ recombinant enzyme, pH 9.5, 50┬░C Bacillus methanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus methanolicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ bound to enzyme, essential for binding of NAD+ Bacillus methanolicus
Zn2+ bound to enzyme Bacillus methanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
10 * 43000 Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Bacillus methanolicus
-
formaldehyde + NADH + H+
-
?
methanol + NAD+ Bacillus methanolicus C1
-
formaldehyde + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
-
-
Bacillus methanolicus C1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant proteins Bacillus methanolicus

Reaction

Reaction Comment Organism Reaction ID
methanol + NAD+ = formaldehyde + NADH + H+ ping-pong mechanism in the absence of ACT protein Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + NAD+
-
Bacillus methanolicus formaldehyde + NADH + H+
-
?
methanol + NAD+
-
Bacillus methanolicus C1 formaldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
decamer 10 * 43000 Bacillus methanolicus

Synonyms

Synonyms Comment Organism
More not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8 Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+ tightly but not covalentely bound to enzyme Bacillus methanolicus