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Literature summary for 1.1.1.244 extracted from

  • Arfman, N.; Dijkhuizen, L.
    Methanol dehydrogenase from thermotolerant methylotroph Bacillus C1 (1990), Methods Enzymol., 188, 223-226.
    View publication on PubMed

General Stability

General Stability Organism
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation Bacillus methanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus methanolicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
10 * 43000, SDS-PAGE Bacillus methanolicus
363000
-
ultracentrifugation sedimentation equilibrium, dissociation occurs during the centrifugation time of 80 h Bacillus methanolicus
430000
-
calculated from electron microscopic analysis and MW of subunit Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
thermotolerant methylotrophic strain C1
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered Bacillus methanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
19.6
-
formaldehyde reductase activity Bacillus methanolicus

Storage Stability

Storage Stability Organism
-80 to 4┬░C, in the presence of DTT at least 4 days stable Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+ best substrate Bacillus methanolicus ethanal + NADH
-
?
methanol + NAD+ 44% of activity with ethanol, NADP+ cannot replace NAD+ Bacillus methanolicus formaldehyde + NADH + H+
-
?
methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
r
n-butanol + NAD+ 87% of activity with ethanol Bacillus methanolicus n-butanal + NADH
-
?
n-propanol + NAD+ 71% of activity with ethanol Bacillus methanolicus n-propanal + NADH
-
?

Subunits

Subunits Comment Organism
decamer 10 * 43000, SDS-PAGE Bacillus methanolicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
formaldehyde reduction Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+ required, specific for Bacillus methanolicus