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Literature summary for 1.1.1.244 extracted from

  • Arfman, N.; Watling, E.M.; Clement, W.; Van Oosterwijk, R.J.; De Vries, G.E.; Harder, W.; Attwood, M.M.; Dijkhuizen, L.
    Methanol metabolism in thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase as a key enzyme (1989), Arch. Microbiol., 152, 280-288.
    View publication on PubMed

General Stability

General Stability Organism
dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde Bacillus methanolicus
2
-
formaldehyde
-
Bacillus methanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus methanolicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
10 * 43000, SDS-PAGE Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Bacillus methanolicus
-
ethanal + NADH
-
?
methanol + NAD+ Bacillus methanolicus involved in initial methanol oxidation formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
thermotolerant methylotrophic strain C1
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered Bacillus methanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
19.6
-
formaldehyde reductase activity Bacillus methanolicus

Storage Stability

Storage Stability Organism
-80 to 4┬░C, in the presence of DTT at least 4 days stable Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Bacillus methanolicus ethanal + NADH
-
?
ethanol + NAD+ best substrate Bacillus methanolicus ethanal + NADH
-
r
methanol + NAD+ 33% of activity with ethanol Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+ involved in initial methanol oxidation Bacillus methanolicus formaldehyde + NADH + H+
-
r
n-butanol + NAD+ 87% of activity with ethanol Bacillus methanolicus n-butanal + NADH
-
r
n-propanol + NAD+ 71% of activity with ethanol Bacillus methanolicus n-propanal + NADH
-
r

Subunits

Subunits Comment Organism
decamer 10 * 43000, SDS-PAGE Bacillus methanolicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
formaldehyde reduction Bacillus methanolicus
9.5
-
methanol oxidation Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
additional information no pyrroloquinoline quinone enzyme Bacillus methanolicus
NAD+ required, specific for Bacillus methanolicus