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Literature summary for 1.1.1.244 extracted from

  • Le, T.K.; Ju, S.B.; Lee, H.W.; Lee, J.Y.; Oh, S.H.; Kwon, K.K.; Sung, B.H.; Lee, S.G.; Yeom, S.J.
    Biosensor-based directed evolution of methanol dehydrogenase from Lysinibacillus xylanilyticus (2021), Int. J. Mol. Sci., 22, 1471 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E396V mutant enzyme has superior methanol conversion efficiency, with 79fold improvements compared to the wild-type Lysinibacillus xylanilyticus
E396V mutant enzyme shows high activity, particularly at very low methanol concentrations Lysinibacillus xylanilyticus
K318N mutant enzyme has superior methanol conversion efficiency, with 23fold improvements compared to the wild-type Lysinibacillus xylanilyticus
K318N mutant enzyme shows high activity, particularly at very low methanol concentrations Lysinibacillus xylanilyticus
K46E mutant enzyme has superior methanol conversion efficiency, with 3fold improvements compared to the wild-type Lysinibacillus xylanilyticus
K46E mutant enzyme shows high activity, particularly at very low methanol concentrations Lysinibacillus xylanilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V Lysinibacillus xylanilyticus
0.046
-
methanol pH 9.5, 55┬░C, mutant enzyme K318N Lysinibacillus xylanilyticus
0.233
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V/K318N Lysinibacillus xylanilyticus
0.372
-
methanol pH 9.5, 55┬░C, mutant enzyme K46E Lysinibacillus xylanilyticus
1.311
-
methanol pH 9.5, 55┬░C, wild-type enzyme Lysinibacillus xylanilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Lysinibacillus xylanilyticus
-
formaldehyde + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Lysinibacillus xylanilyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysinibacillus xylanilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + NAD+
-
Lysinibacillus xylanilyticus formaldehyde + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
lxmdh
-
Lysinibacillus xylanilyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V Lysinibacillus xylanilyticus
0.022
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V/K318N Lysinibacillus xylanilyticus
0.027
-
methanol pH 9.5, 55┬░C, mutant enzyme K318N Lysinibacillus xylanilyticus
0.028
-
methanol pH 9.5, 55┬░C, mutant enzyme K46E Lysinibacillus xylanilyticus
0.0333
-
methanol pH 9.5, 55┬░C, wild-type enzyme Lysinibacillus xylanilyticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lysinibacillus xylanilyticus

General Information

General Information Comment Organism
metabolism methanol dehydrogenase, is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy Lysinibacillus xylanilyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.025
-
methanol pH 9.5, 55┬░C, wild-type enzyme Lysinibacillus xylanilyticus
0.076
-
methanol pH 9.5, 55┬░C, mutant enzyme K46E Lysinibacillus xylanilyticus
0.093
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V/K318N Lysinibacillus xylanilyticus
0.58
-
methanol pH 9.5, 55┬░C, mutant enzyme K318N Lysinibacillus xylanilyticus
2
-
methanol pH 9.5, 55┬░C, mutant enzyme E396V Lysinibacillus xylanilyticus