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Literature summary for 1.1.1.244 extracted from

  • Roth, T.B.; Woolston, B.M.; Stephanopoulos, G.; Liu, D.R.
    Phage-assisted evolution of Bacillus methanolicus methanol dehydrogenase 2 (2019), ACS Synth. Biol., 8, 796-806 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A164P 3fold increase in kcat/Km as compared to wild-type enzyme Bacillus methanolicus
A164P 4.7fold increase in kcat/Km as compared to wild-type enzyme Bacillus methanolicus
A363L 5.1fold increase in kcat/Km as compared to wild-type enzyme Bacillus methanolicus
E123G 1.1fold increase in kcat/Km as compared to wild-type enzyme Bacillus methanolicus
M163V 1.5fold increase in kcat/Km as compared to wild-type enzyme Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
329
-
methanol pH 9.5, 37┬░C, mutant enzyme A164P/A363L Bacillus methanolicus
432
-
methanol pH 9.5, 37┬░C, mutant enzyme A363L Bacillus methanolicus
440
-
methanol pH 9.5, 37┬░C, mutant enzyme A164P Bacillus methanolicus
615
-
methanol pH 9.5, 37┬░C, mutant enzyme E123G Bacillus methanolicus
627
-
methanol pH 9.5, 37┬░C, mutant enzyme M163V Bacillus methanolicus
636
-
methanol pH 9.5, 37┬░C, wild-type enzyme Bacillus methanolicus

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus I3E2P9
-
-
Bacillus methanolicus ATCC 53907 I3E2P9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + NAD+
-
Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Bacillus methanolicus ATCC 53907 formaldehyde + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
MDH2
-
Bacillus methanolicus
methanol dehydrogenase 2
-
Bacillus methanolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00023
-
methanol pH 9.5, 37┬░C, wild-type enzyme Bacillus methanolicus
0.00025
-
methanol pH 9.5, 37┬░C, mutant enzyme E123G Bacillus methanolicus
0.00035
-
methanol pH 9.5, 37┬░C, mutant enzyme M163V Bacillus methanolicus
0.00069
-
methanol pH 9.5, 37┬░C, mutant enzyme A164P Bacillus methanolicus
0.00108
-
methanol pH 9.5, 37┬░C, mutant enzyme A164P/A363L Bacillus methanolicus
0.00118
-
methanol pH 9.5, 37┬░C, mutant enzyme A363L Bacillus methanolicus