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Literature summary for 1.1.1.244 extracted from

  • Krog, A.; Heggeset, T.M.; Mueller, J.E.; Kupper, C.E.; Schneider, O.; Vorholt, J.A.; Ellingsen, T.E.; Brautaset, T.
    Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties (2013), PLoS ONE, 8, e59188.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
activator protein ACT all three isozymes are activated by ACT, which is encoded by gene act, overview Bacillus methanolicus
NudF protein can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro Bacillus methanolicus

Cloned(Commentary)

Cloned (Comment) Organism
three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566 Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh3 Bacillus methanolicus
1.1
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh1 Bacillus methanolicus
3
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh1 Bacillus methanolicus
4.5
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh2 Bacillus methanolicus
7
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh2 Bacillus methanolicus
7.1
-
formaldehyde pH 6.7, 45┬░C, isozyme Mdh3 Bacillus methanolicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Bacillus methanolicus
-
formaldehyde + NADH + H+
-
r
methanol + NAD+ Bacillus methanolicus PB1
-
formaldehyde + NADH + H+
-
r
methanol + NAD+ Bacillus methanolicus MGA3
-
formaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Bacillus methanolicus
-
NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Bacillus methanolicus MGA3
-
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-
Bacillus methanolicus PB1
-
NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propandiol + NAD+ very low activity Bacillus methanolicus ? + NADH + H+
-
?
1,3-propandiol + NAD+ low activity Bacillus methanolicus ? + NADH + H+
-
?
butanol + NAD+
-
Bacillus methanolicus butanal + NADH + H+
-
?
ethanol + NAD+
-
Bacillus methanolicus acetaldehyde + NADH + H+
-
r
formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus methanol + NAD+
-
r
formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 methanol + NAD+
-
r
formaldehyde + NADH + H+ Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 methanol + NAD+
-
r
isopropanol + NAD+ low activity Bacillus methanolicus isopropanal + NADH + H+
-
?
methanol + NAD+
-
Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus PB1 formaldehyde + NADH + H+
-
r
methanol + NAD+
-
Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
r
methanol + NAD+ very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol Bacillus methanolicus MGA3 formaldehyde + NADH + H+
-
r
additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus ?
-
?
additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus PB1 ?
-
?
additional information all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes Bacillus methanolicus MGA3 ?
-
?
propanol + NAD+
-
Bacillus methanolicus propanal + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
MDH
-
Bacillus methanolicus
NAD+ dependent methanol dehydrogenase
-
Bacillus methanolicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
activity optimum at about 45┬░C for all isozymes Bacillus methanolicus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
20 min, the isozymes are all stable up to Bacillus methanolicus
45
-
20 min, the isozymes are stable up to, except for isozyme Mdh1, which shows about 35-40% loss of activity Bacillus methanolicus
60
-
20 min, isozyme Mdh1 is inactivated, isozyme Mdh2 loses about 80% of activity, while isozyme Mdh3 retains over 80% of its activity Bacillus methanolicus
60
-
20 min, isozyme Mdh1 loses 90% activity, isozyme Mdh2 loses 80% of activity, while isozyme Mdh3 retains 60% of its activity Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
formaldehyde reduction assay at Bacillus methanolicus
9.5
-
alcohol oxidation reaction assay at Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Bacillus methanolicus
NADH
-
Bacillus methanolicus

General Information

General Information Comment Organism
evolution strain MGA3 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes Bacillus methanolicus
evolution strain PB1 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes Bacillus methanolicus
physiological function biological significance of Mdh for methanol oxidation during methylotrophic growth and biological role of the enzyme as part of a formaldehyde detoxification system in the methanol consuming cells Bacillus methanolicus