KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
(4R,6S)-carveol | - |
Rhodococcus erythropolis | |
0.35 | - |
(4R,6R)-carveol | - |
Rhodococcus erythropolis | |
0.39 | - |
(4S,6S)-carveol | - |
Rhodococcus erythropolis | |
2 | - |
(4S,6R)-carveol | - |
Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Rhodococcus erythropolis |
120000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R,6S)-trans-carveol + NAD+ | Rhodococcus erythropolis | enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known | (4R)-carvone + NADH | - |
ir | |
(4R,6S)-trans-carveol + NAD+ | Rhodococcus erythropolis DCL14 | enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known | (4R)-carvone + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
enzyme is induced after growth on limonene or carveol | - |
Rhodococcus erythropolis DCL14 | - |
enzyme is induced after growth on limonene or carveol | - |
Purification (Comment) | Organism |
---|---|
octyl Sepharose, gel filtration, hydroxyapatite, anion exchange | Rhodococcus erythropolis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+ | steroselective conversion of (6S)-carveol stereomers from a diastereomeric mixture of (4R)- or (4S)-carveol | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.46 | - |
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,2R,4S)-limonene-1,2-diol + NAD+ | 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | ? | - |
ir | |
(1S,2S,4R)-limonene-1,2-diol + NAD+ | 25% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | ? | - |
ir | |
(4R,6R)-carveol + NADP+ | - |
Rhodococcus erythropolis | ? | - |
r | |
(4R,6S)-dihydrocarveol + NAD+ | 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | (4R)-carvone + NADH | - |
ir | |
(4R,6S)-trans-carveol + NAD+ | 95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | (4R)-carvone + NADH | - |
ir | |
(4R,6S)-trans-carveol + NAD+ | enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known | Rhodococcus erythropolis | (4R)-carvone + NADH | - |
ir | |
(4R,6S)-trans-carveol + NAD+ | 95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis DCL14 | (4R)-carvone + NADH | - |
ir | |
(4R,6S)-trans-carveol + NAD+ | enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known | Rhodococcus erythropolis DCL14 | (4R)-carvone + NADH | - |
ir | |
(4S,6R)-carveol + NADP+ | - |
Rhodococcus erythropolis | ? | - |
r | |
(4S,6RS)-carveol + NAD+ | enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | (4S)-carvone + NADH | - |
ir | |
(4S,6RS)-carveol + NAD+ | enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis DCL14 | (4S)-carvone + NADH | - |
ir | |
(4S,6S)-carveol + NADP+ | - |
Rhodococcus erythropolis | ? | - |
r | |
(4S,6S)-dihydrocarveol + NAD+ | 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | (4S)-carvone + NADH | - |
ir | |
(R)-indanol + NAD+ | 13% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | indanone + NADH | - |
ir | |
(S)-indanol + NAD+ | 30% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | indanon + NADH | - |
ir | |
2-ethylcyclohexanol + NAD+ | 110% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 2-ethylcyclohexanone + NADH | - |
ir | |
2-methylcyclohexanol + NAD+ | 100% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 2-methylcyclohexanone + NADH | - |
ir | |
3,3,5,5-tetramethylcyclohexanol + NAD+ | 55% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 3,3,5,5-tetramethylcyclohexanone + NADH | - |
ir | |
3,5,5-trimethylcyclohex-2-en-1-ol + NAD+ | 115% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 3,5,5-trimethylcyclohex-2-en-1-one + NADH | - |
ir | |
3-methylcyclohexanol + NAD+ | 165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 3-methylcyclohexanone + NADH | - |
ir | |
3-methylcyclohexanol + NAD+ | 165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis DCL14 | 3-methylcyclohexanone + NADH | - |
ir | |
4-ethylcyclohexanol + NAD+ | 40% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 4-ethylcyclohexanone + NADH | - |
ir | |
4-methylcyclohexanol + NAD+ | 35% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | 4-methylcyclohexanone + NADH | - |
ir | |
cyclohex-2-ene-1-ol + NAD+ | 10% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | cyclohex-2-ene-1-one + NADH | - |
ir | |
cyclohexanol + NAD+ | 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | cyclohexanone + NADH | - |
ir | |
cyclohexanol + NAD+ | 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis DCL14 | cyclohexanone + NADH | - |
ir | |
sobrerol + NAD+ | 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor | Rhodococcus erythropolis | ? | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | 50 | gradual increase in specific activity from 0.55 micromol/min mg to 2.85 micromol/min mg | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
time dependent inactivation above | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
25% of optimal activity at pH 4.6 and 7.0 | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | each monomer of carveol dehydrogenase contains one tightly bound NAD(H) molecule | Rhodococcus erythropolis |