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Literature summary for 1.1.1.240 extracted from

  • Horiuchi, T.; Kurokawa, T.
    Purification and characterization of N-acetyl-D-hexosamine dehydrogenase from Pseudomonas sp. No. 53 (1989), Agric. Biol. Chem., 53, 1919-1925.
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Search for more literature for 1.1.1.240

General Stability

General Stability Organism
stabilized against heating by 3 mM NAD+ Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
NaN3 weak Pseudomonas sp.
SDS
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 NAD+ with N-acetyl-D-glucosamine as substrate Pseudomonas sp.
0.8
-
 N-acetyl-D-galactosamine
-
 Pseudomonas sp.
5.3
-
 N-acetyl-D-glucosamine
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Hg2+ inhibitory Pseudomonas sp.
additional information not effected by Ca2+, Mg2+, Cd2+, Ni2+, Zn2+, Cu2+, Mn2+, Co2+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Pseudomonas sp.
124000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine + NAD+ Pseudomonas sp.
-
 N-acetyl-D-glucosaminate + NADH
-
 ?
N-acetyl-D-glucosamine + NAD+ Pseudomonas sp. No. 53
-
 N-acetyl-D-glucosaminate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 No. 53
-
Pseudomonas sp. No. 53
-
 No. 53
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.265
-
 crude extract Pseudomonas sp.
239
-
 purified enzyme Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, several months, without much loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-galactosamine + NAD+
-
 Pseudomonas sp. N-acetyl-D-galactoseaminate + NADH
-
 ?
N-acetyl-D-galactosamine + NAD+
-
 Pseudomonas sp. No. 53 N-acetyl-D-galactoseaminate + NADH
-
 ?
N-acetyl-D-glucosamine + NAD+
-
 Pseudomonas sp. N-acetyl-D-glucosaminate + NADH
-
 ?
N-acetyl-D-glucosamine + NAD+
-
 Pseudomonas sp. No. 53 N-acetyl-D-glucosaminate + NADH
-
 ?
N-acetyl-D-mannosamine + NAD+ + H2O very poor substrate Pseudomonas sp. N-acetyl-D-mannosaminate + NADH + H+
-
 ?
N-acetyl-D-mannosamine + NAD+ + H2O very poor substrate Pseudomonas sp. No. 53 N-acetyl-D-mannosaminate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 30000, SDS-PAGE, gel filtration Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, unstable below pH 5 and above pH 12 Pseudomonas sp.
55
-
 10 min, stable Pseudomonas sp.
65
-
 10 min, 50% loss of activity Pseudomonas sp.
70
-
 10 min, complete loss of activity Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
655
-
 N-acetyl-D-glucosamine
-
 Pseudomonas sp.
932
-
 N-acetyl-D-galactosamine
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10.5 most stable Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ strictly specific for NAD+ as hydrogen acceptor Pseudomonas sp.