Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockout results in loss of the ability to grow on quinate and/or shikimate | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloromercuribenzene 4-sulfonate | inhibits both activities of the enzyme | Aspergillus niger | |
additional information | the enzyme is strongly repressed by D-glucose | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
NAD+ | - |
Aspergillus niger | |
0.26 | - |
NAD+ | + quinate | Aspergillus niger | |
0.32 | 0.37 | quinate | - |
Aspergillus niger | |
0.42 | - |
NAD+ | + shikimate | Aspergillus niger | |
6.25 | - |
quinate | - |
Aspergillus niger | |
11.5 | - |
shikimate | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | Aspergillus niger | - |
3-dehydroquinate + NADH + H+ | - |
r | |
additional information | Aspergillus niger | addition of either shikimate or quinate to the assay does not increase the reaction rate when the other substrate is present alone | ? | - |
? | |
shikimate + NAD+ | Aspergillus niger | - |
3-dehydroshikimate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger | - |
bifunctional enzyme: quinate (shikimate) dehydrogenase, different from EC 1.1.1.25 | - |
Purification (Comment) | Organism |
---|---|
separation from NADP+-linked shikimate dehydrogenase, EC 1.1.1.25, gel filtration | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | - |
Aspergillus niger | 3-dehydroquinate + NADH + H+ | - |
r | |
additional information | addition of either shikimate or quinate to the assay does not increase the reaction rate when the other substrate is present alone | Aspergillus niger | ? | - |
? | |
additional information | the bifunctional enzyme shows quinate and shikimate dehydrogenase activities | Aspergillus niger | ? | - |
? | |
quinate + NAD+ | - |
Aspergillus niger | 5-dehydroquinate + NADH + H+ | - |
? | |
shikimate + NAD+ | - |
Aspergillus niger | ? | - |
? | |
shikimate + NAD+ | - |
Aspergillus niger | 3-dehydroshikimate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
quinate dehydrogenase | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
inactivation, kinetics vary in presence of quinate or shikimate, respectively | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
different pH optima for shikimate and quinate | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP(H) | Aspergillus niger | |
NAD+ | - |
Aspergillus niger | |
NADH | - |
Aspergillus niger |