BRENDA - Enzyme Database
show all sequences of 1.1.1.239

Characterization of hamster NAD+-dependent 3(17)beta-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily

Endo, S.; Noda, M.; Ikari, A.; Tatematsu, K.; El-Kabbani, O.; Hara, A.; Kitade, Y.; Matsunaga, T.; J. Biochem. 158, 425-434 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Mesocricetus auratus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hexestrol
-
Mesocricetus auratus
hinokitiol
-
Mesocricetus auratus
additional information
no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone
Mesocricetus auratus
Phenolphthalein
-
Mesocricetus auratus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Mesocricetus auratus
0.0012
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0097
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.011
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.013
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.013
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.013
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
0.03
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.061
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.076
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.095
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.148
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.162
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.181
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.569
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.63
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.38
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
recombinant His-tagged enzyme, SDS-PAGE
Mesocricetus auratus
Organism
Organism
UniProt
Commentary
Textmining
Mesocricetus auratus
A0A097ZMY7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis
Mesocricetus auratus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
in male hamsters
Mesocricetus auratus
-
colon
in male hamsters
Mesocricetus auratus
-
heart
in female hamsters
Mesocricetus auratus
-
kidney
in male hamsters
Mesocricetus auratus
-
liver
-
Mesocricetus auratus
-
additional information
mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters
Mesocricetus auratus
-
small intestine
in male hamsters
Mesocricetus auratus
-
testis
in male hamsters
Mesocricetus auratus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-1,2,3,4-tetrahydro-1-naphthol + NAD+
i.e. (R)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
-
-
?
(S)-1,2,3,4-tetrahydro-1-naphthol + NAD+
i.e. (S)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
-
-
?
(S)-1,2,3,4-tetrahydro-1-naphthol + NADP+
i.e. (S)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADPH + H+
-
-
-
?
(S)-1-indanol + NAD+
-
740637
Mesocricetus auratus
2,3-dihydro-1H-inden-1-one + NADH + H+
-
-
-
?
(S)-camphorquinone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
(S)-camphorquinone + NADPH + H+
-
740637
Mesocricetus auratus
? + NADP+
-
-
-
?
1-acenaphthenol + NAD+
-
740637
Mesocricetus auratus
acenaphthylen-1(2H)-one + NADH + H+
-
-
-
?
17beta-estradiol + NAD+
-
740637
Mesocricetus auratus
estrone + NADH + H+
-
-
-
?
2-cyclohexen-1-ol + NAD+
-
740637
Mesocricetus auratus
2-cyclohexen-1-one + NADH + H+
-
-
-
?
20alpha-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
3alpha-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
3beta-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-androstan-3alpha-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5alpha-androstan-3,20-dione + NADH + H+
-
-
-
?
5alpha-androstan-3beta-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5alpha-androstan-3,17-dione + NADH + H+
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-androstane-3beta,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-dihydrotestosterone + NADH + H+
-
740637
Mesocricetus auratus
testosterone + NADH + H+
-
-
-
?
5alpha-pregnan-20alpha-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5alpha-pregnan-3beta-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH + H+
-
-
-
?
5beta-androstan-3alpha-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH + H+
-
-
-
?
5beta-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5beta-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5beta-androstane-3beta,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5beta-dihydrotestosterone + NAD+
-
740637
Mesocricetus auratus
testosterone + NADH + H+
-
-
-
?
5beta-pregnan-20alpha-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-pregnan-3alpha-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-pregnan-3beta,21-diol-20-one + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5beta-pregnan-3beta-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
alpha-3-hydroxyhexobarbital + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
beta-3-hydroxyhexobarbital + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
beta-ionol + NAD+
-
740637
Mesocricetus auratus
(3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+
-
-
-
?
cis-benzene dihydrodiol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
dehydroepiandrosterone + NAD+
-
740637
Mesocricetus auratus
? + NADH + H+
-
-
-
?
farnesol + NAD+
-
740637
Mesocricetus auratus
farnesal + NADH + H+
-
-
-
?
geraniol + NAD+
-
740637
Mesocricetus auratus
geranial + NADH + H+
-
-
-
?
geranylgeraniol + NAD+
-
740637
Mesocricetus auratus
geranylgeranial + NADH + H+
-
-
-
?
additional information
the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, substrate specificity, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). AKR1C34 also has morphine dehydrogenase activity, EC 1.1.1.218. No activity with cyclohexanol
740637
Mesocricetus auratus
?
-
-
-
?
nerol + NAD+
-
740637
Mesocricetus auratus
neral + NADH + H+
-
-
-
?
testosterone + NAD+
-
740637
Mesocricetus auratus
4-androstenedione + NADPH + H+
-
-
-
?
trans-benzene dihydrodiol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 37000, recombinant His-tagged enzyme, SDS-PAGE
Mesocricetus auratus
Synonyms
Synonyms
Commentary
Organism
AKR1C34
-
Mesocricetus auratus
More
cf. EC 1.1.1.218, morphine 6-dehydrogenase
Mesocricetus auratus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Mesocricetus auratus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0045
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0075
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0153
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.025
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
0.028
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.032
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.038
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.04
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.045
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.053
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0917
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.0967
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.1
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.105
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.115
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.167
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.283
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
substrate oxidation
Mesocricetus auratus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme shows high preference for NAD+ compared with NADP+
Mesocricetus auratus
NAD+
conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity
Mesocricetus auratus
NADH
-
Mesocricetus auratus
NADP+
-
Mesocricetus auratus
NADPH
-
Mesocricetus auratus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00065
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
Phenolphthalein
0.0032
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
hinokitiol
0.013
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
Hexestrol
Cloned(Commentary) (protein specific)
Commentary
Organism
gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
Mesocricetus auratus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme shows high preference for NAD+ compared with NADP+
Mesocricetus auratus
NAD+
conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity
Mesocricetus auratus
NADH
-
Mesocricetus auratus
NADP+
-
Mesocricetus auratus
NADPH
-
Mesocricetus auratus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00065
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
Phenolphthalein
0.0032
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
hinokitiol
0.013
-
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
Hexestrol
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hexestrol
-
Mesocricetus auratus
hinokitiol
-
Mesocricetus auratus
additional information
no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone
Mesocricetus auratus
Phenolphthalein
-
Mesocricetus auratus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Mesocricetus auratus
0.0012
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0097
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.011
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.013
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.013
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.013
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
0.03
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.061
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.076
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.095
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.148
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.162
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.181
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.569
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.63
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.38
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
recombinant His-tagged enzyme, SDS-PAGE
Mesocricetus auratus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis
Mesocricetus auratus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
in male hamsters
Mesocricetus auratus
-
colon
in male hamsters
Mesocricetus auratus
-
heart
in female hamsters
Mesocricetus auratus
-
kidney
in male hamsters
Mesocricetus auratus
-
liver
-
Mesocricetus auratus
-
additional information
mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters
Mesocricetus auratus
-
small intestine
in male hamsters
Mesocricetus auratus
-
testis
in male hamsters
Mesocricetus auratus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-1,2,3,4-tetrahydro-1-naphthol + NAD+
i.e. (R)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
-
-
?
(S)-1,2,3,4-tetrahydro-1-naphthol + NAD+
i.e. (S)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADH + H+
-
-
-
?
(S)-1,2,3,4-tetrahydro-1-naphthol + NADP+
i.e. (S)-tetralol
740637
Mesocricetus auratus
3,4-dihydronaphthalen-1(2H)-one + NADPH + H+
-
-
-
?
(S)-1-indanol + NAD+
-
740637
Mesocricetus auratus
2,3-dihydro-1H-inden-1-one + NADH + H+
-
-
-
?
(S)-camphorquinone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
(S)-camphorquinone + NADPH + H+
-
740637
Mesocricetus auratus
? + NADP+
-
-
-
?
1-acenaphthenol + NAD+
-
740637
Mesocricetus auratus
acenaphthylen-1(2H)-one + NADH + H+
-
-
-
?
17beta-estradiol + NAD+
-
740637
Mesocricetus auratus
estrone + NADH + H+
-
-
-
?
2-cyclohexen-1-ol + NAD+
-
740637
Mesocricetus auratus
2-cyclohexen-1-one + NADH + H+
-
-
-
?
20alpha-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
3alpha-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
3beta-hydroxyprogesterone + NADH + H+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-androstan-3alpha-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5alpha-androstan-3,20-dione + NADH + H+
-
-
-
?
5alpha-androstan-3beta-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5alpha-androstan-3,17-dione + NADH + H+
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5alpha-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-androstane-3beta,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5alpha-dihydrotestosterone + NADH + H+
-
740637
Mesocricetus auratus
testosterone + NADH + H+
-
-
-
?
5alpha-pregnan-20alpha-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5alpha-pregnan-3beta-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5alpha-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-androstan-17beta-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH + H+
-
-
-
?
5beta-androstan-3alpha-ol-17-one + NAD+
-
740637
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH + H+
-
-
-
?
5beta-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5beta-androstane-3alpha,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5beta-androstane-3beta,17beta-diol + NAD+
-
740637
Mesocricetus auratus
? + NAD+
-
-
-
?
5beta-dihydrotestosterone + NAD+
-
740637
Mesocricetus auratus
testosterone + NADH + H+
-
-
-
?
5beta-pregnan-20alpha-ol-3-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-pregnan-3alpha-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
5beta-pregnan-3beta,21-diol-20-one + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
5beta-pregnan-3beta-ol-20-one + NAD+
-
740637
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH + H+
-
-
-
?
alpha-3-hydroxyhexobarbital + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
beta-3-hydroxyhexobarbital + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
beta-ionol + NAD+
-
740637
Mesocricetus auratus
(3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+
-
-
-
?
cis-benzene dihydrodiol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
dehydroepiandrosterone + NAD+
-
740637
Mesocricetus auratus
? + NADH + H+
-
-
-
?
farnesol + NAD+
-
740637
Mesocricetus auratus
farnesal + NADH + H+
-
-
-
?
geraniol + NAD+
-
740637
Mesocricetus auratus
geranial + NADH + H+
-
-
-
?
geranylgeraniol + NAD+
-
740637
Mesocricetus auratus
geranylgeranial + NADH + H+
-
-
-
?
additional information
the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, substrate specificity, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). AKR1C34 also has morphine dehydrogenase activity, EC 1.1.1.218. No activity with cyclohexanol
740637
Mesocricetus auratus
?
-
-
-
?
nerol + NAD+
-
740637
Mesocricetus auratus
neral + NADH + H+
-
-
-
?
testosterone + NAD+
-
740637
Mesocricetus auratus
4-androstenedione + NADPH + H+
-
-
-
?
trans-benzene dihydrodiol + NAD+
-
740637
Mesocricetus auratus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, recombinant His-tagged enzyme, SDS-PAGE
Mesocricetus auratus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Mesocricetus auratus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0045
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0075
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0153
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.025
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
0.028
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.032
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.038
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.04
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.045
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.053
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.0917
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.0967
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.1
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.105
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.115
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.167
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.283
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
substrate oxidation
Mesocricetus auratus
General Information
General Information
Commentary
Organism
evolution
enzyme AKR1C34 is a member of the aldo–keto reductase (AKR) 1C subfamily in the AKR superfamily
Mesocricetus auratus
physiological function
enzyme AKR1C34 oxidizes various xenobiotic alicyclic alcohols using NAD+
Mesocricetus auratus
General Information (protein specific)
General Information
Commentary
Organism
evolution
enzyme AKR1C34 is a member of the aldo–keto reductase (AKR) 1C subfamily in the AKR superfamily
Mesocricetus auratus
physiological function
enzyme AKR1C34 oxidizes various xenobiotic alicyclic alcohols using NAD+
Mesocricetus auratus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.05
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.05
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.067
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.067
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.083
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.2
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.42
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.55
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.67
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.83
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
2
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
2.17
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
5.3
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
7.5
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
12.67
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
33.3
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.05
-
trans-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.05
-
2-Cyclohexen-1-ol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.05
-
cis-benzene dihydrodiol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.067
-
nerol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.067
-
NADP+
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
0.083
-
(R)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.2
-
geraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.42
-
farnesol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
0.55
-
(S)-1-indanol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.67
-
(S)-1,2,3,4-tetrahydro-1-naphthol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
1.83
-
1-Acenaphthenol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
2
-
NAD+
pH 7.4, 25°C, with substrate S-tetralol
Mesocricetus auratus
2.17
-
alpha-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
5.3
-
beta-ionol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
7.5
-
beta-3-hydroxyhexobarbital
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
12.67
-
NADH
pH 7.4, 25°C, with substrate S-camphorquinone
Mesocricetus auratus
33.3
-
geranylgeraniol
pH 7.4, 25°C, recombinant enzyme
Mesocricetus auratus
Other publictions for EC 1.1.1.239
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740293
Zang
Screening baccharin analogs as ...
Homo sapiens
Chem. Biol. Interact.
234
339-348
2015
-
-
-
-
-
-
17
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
3
1
-
-
-
1
-
-
2
-
-
15
-
-
-
2
-
-
-
15
17
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
740637
Endo
Characterization of hamster NA ...
Mesocricetus auratus
J. Biochem.
158
425-434
2015
-
-
1
-
-
-
4
18
1
-
1
-
-
1
-
-
1
-
-
8
-
-
43
1
2
1
-
-
17
1
-
-
5
-
-
3
-
-
1
5
-
-
-
3
4
-
18
1
-
1
-
-
-
-
1
-
8
-
-
43
1
1
-
-
17
1
-
-
-
-
2
2
-
17
17
739864
Matsunaga
Induction of aldo-keto reducta ...
Homo sapiens
Anticancer Drugs
25
868-877
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
6
-
-
2
-
3
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
2
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
724719
Matsunaga
Pathophysiological roles of al ...
Homo sapiens
Chem. Biol. Interact.
202
234-242
2013
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
1
1
1
1
1
724915
Heinrich
Synthesis and structure-activi ...
Homo sapiens
Eur. J. Med. Chem.
62
738-744
2013
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724916
Gazvoda
2,3-diarylpropenoic acids as s ...
Homo sapiens
Eur. J. Med. Chem.
62
89-97
2013
-
-
-
1
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725674
Liedtke
Development of potent and sele ...
Homo sapiens
J. Med. Chem.
56
2429-2446
2013
-
-
-
1
-
-
71
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
3
-
-
-
-
-
-
-
2
-
-
65
-
-
-
2
1
-
-
65
71
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739953
Endo
Characterization of rabbit mor ...
Oryctolagus cuniculus
Arch. Biochem. Biophys.
529
131-139
2013
-
-
5
-
7
-
10
88
-
-
-
2
-
4
-
-
2
-
-
12
-
-
120
-
17
3
-
-
86
8
-
-
10
6
-
40
-
-
5
10
-
7
-
40
38
6
88
-
-
-
2
-
-
-
2
-
12
-
-
120
-
3
-
-
86
8
-
-
-
-
8
8
-
-
-
723866
Jackson
Structure of AKR1C3 with 3-phe ...
Homo sapiens
Acta Crystallogr. Sect. F
68
409-413
2012
-
-
1
1
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724533
Chen
Crystal structures of AKR1C3 c ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
3492-3497
2012
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724538
Sinreih
N-Benzoyl anthranilic acid der ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
5948-5951
2012
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725153
Miller
Aldo-keto reductase family 1 m ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
5
278-289
2012
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725671
Brozic
Selective inhibitors of aldo-k ...
Homo sapiens
J. Med. Chem.
55
7417-7424
2012
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725825
Byrns
Overexpression of aldo-keto re ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
130
7-15
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
724517
Adeniji
Discovery of substituted 3-(ph ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
1464-1468
2011
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711670
Guise
The bioreductive prodrug PR-10 ...
Homo sapiens
Cancer Res.
70
1573-1584
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
712882
Byrns
Aldo-keto reductase 1C3 expres ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
118
177-187
2010
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
713574
Ashley
Developmental evaluation of al ...
Homo sapiens
Urology
76
67-72
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
725152
Zakharov
Suppressed expression of type ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
3
608-617
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
712571
Nakamura
Type 5 17ß-hydroxysteroid deh ...
Homo sapiens
J. Clin. Endocrinol. Metab.
94
2192-2198
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
-
2
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
10
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670259
Ngatcha
3beta-Alkyl-androsterones as i ...
Homo sapiens
Mol. Cell. Endocrinol.
248
225-232
2006
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673341
Fung
Increased expression of type 2 ...
Homo sapiens
Endocr. Relat. Cancer
13
169-180
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287209
Krazeisen
Phytoestrogens inhibit human 1 ...
Homo sapiens
Mol. Cell. Endocrinol.
171
151-162
2001
-
-
1
-
-
-
9
-
1
-
-
3
-
4
-
-
-
-
-
5
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
9
-
-
1
-
-
3
-
-
-
-
-
5
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287210
Stupans
Testosterone dehydrogenase act ...
Homo sapiens, Notamacropus eugenii, Phascolarctos cinereus, Rattus norvegicus
Comp. Biochem. Physiol. B
125
245-250
2000
-
-
-
-
-
-
1
4
4
-
-
4
-
12
-
-
-
-
-
4
7
-
4
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
4
4
-
-
4
-
-
-
-
-
4
7
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287211
Carre
Characterization and solubizat ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
46
265-267
1993
-
-
-
-
-
-
-
2
1
-
-
1
-
1
-
-
-
-
-
1
-
1
2
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
1
-
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287212
Dumont
Expression of human 17beta-hyd ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
41
605-608
1992
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
7
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286355
Sawada
Kinetic and stereochemical cha ...
Mesocricetus auratus
J. Biochem.
109
770-775
1991
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
286354
Ohmura
Demonstration of 3alpha(17beta ...
Mesocricetus auratus
Biochem. J.
266
583-589
1990
-
-
-
-
-
-
11
13
-
-
-
-
-
1
-
-
1
-
-
1
1
1
10
-
-
-
-
1
12
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
11
-
13
-
-
-
-
-
-
-
1
-
1
1
1
10
-
-
-
1
12
1
-
-
-
-
-
-
-
-
-
287213
Itagaki
Purification and characterizat ...
Ilyonectria destructans
J. Biochem.
103
1039-1044
1988
-
-
-
-
-
-
-
6
-
-
2
1
-
1
-
-
1
-
-
-
1
1
3
1
-
-
-
1
-
2
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
2
1
-
-
-
1
-
-
1
1
3
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
287214
Payne
Isolation of novel microbiol 3 ...
Alcaligenes sp.
J. Biol. Chem.
260
13648-13655
1985
-
-
-
-
-
-
-
6
-
-
3
1
-
1
-
-
1
-
-
-
1
1
13
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
3
1
-
-
-
1
-
-
1
1
13
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287215
Blomquist
Microsomal 17beta-hydroxystero ...
Cavia porcellus
J. Steroid Biochem.
8
193-198
1977
-
-
-
-
-
1
1
4
1
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
1
-
1
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287216
Markert
-
Enzyminduktion bei Streptomyce ...
Streptomyces exfoliatus
Hoppe-Seyler's Z. Physiol. Chem.
356
1843-1852
1975
-
-
-
-
-
-
2
3
-
-
1
1
-
1
-
-
1
-
-
-
-
-
8
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
3
-
-
1
1
-
-
-
1
-
-
-
-
8
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
287217
Ghraf
11beta- und 17beta-Hydroxyster ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
354
299-305
1973
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
6
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287218
Endahl
-
Separation of a triphosphopyri ...
Cavia porcellus
J. Biol. Chem.
235
2793-2796
1960
-
-
-
-
-
-
8
1
-
-
-
1
-
1
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287219
Villee
Some properties of the pyridin ...
Cavia porcellus
J. Biol. Chem.
235
3615-3619
1960
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287220
Sweat
Preparation and characterizati ...
Bos taurus
J. Biol. Chem.
185
75-84
1950
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-