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Literature summary for 1.1.1.238 extracted from
- Characterization of NADP-dependent 12beta-hydroxysteroid dehydrogenase from Clostridium paraputrificum (1988), Biochim. Biophys. Acta, 962, 362-370.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | potential application in quantification of 12beta-hydroxyl groups in di- and trisubstituted bile acids | Clostridium paraputrificum |
General Stability
| General Stability | Organism |
|---|---|
| most stable in presence of reducing agents e.g. dithiothreitol | Clostridium paraputrificum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0837 | - |
7,12-Dioxo-5beta-cholanoate | - |
Clostridium paraputrificum |  |
| 0.0854 | - |
NADPH | - |
Clostridium paraputrificum | |
| 0.0895 | - |
3,12-Dioxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
| 0.138 | - |
7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
| 0.141 | - |
3alpha-Hydroxy-12-oxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
| 0.142 | - |
3,7,12-trioxotaureate | - |
Clostridium paraputrificum | |
| 0.144 | - |
3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
| 0.171 | - |
3,7,12-Trioxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
| 0.274 | - |
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate | - |
Clostridium paraputrificum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Clostridium paraputrificum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126000 | - |
gel filtration | Clostridium paraputrificum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium paraputrificum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | 4.4 | depending on substrate | Clostridium paraputrificum |
| 2.2 | 6.4 | depending on inducer | Clostridium paraputrificum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, lyophilized powder, at least 4 weeks | Clostridium paraputrificum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,12-dioxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+ | - |
? | |
| 3,7,12-trioxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 12beta-hydroxy-3,7-dioxo-5beta-cholanoate + NADP+ | - |
? | |
| 3,7,12-trioxotaureate + NADPH | - |
Clostridium paraputrificum | 12beta-hydroxy-3,7-dioxotaureate + NADP+ | - |
? | |
| 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+ | - |
? | |
| 3alpha-hydroxy-12-oxo-5beta-cholanoate + NADPH | reverse reaction 10% the rate of forward reaction | Clostridium paraputrificum | 3alpha,12beta-dihydroxy-5beta-cholanoate + NADP+ | - |
r | |
| 3alpha-hydroxy-7,12-dioxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 3alpha,12beta-dihydroxy-7-oxo-5beta-cholanoate + NADP+ | - |
? | |
| 7,12-dioxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 12beta-hydroxy-7-oxo-5beta-cholanoate + NADP+ | - |
? | |
| 7alpha-hydroxy-3,12-dioxo-5beta-cholanoate + NADPH | - |
Clostridium paraputrificum | 7alpha,12beta-dihydroxy-3-oxo-5beta-cholanoate + NADP+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
at least 6 h | Clostridium paraputrificum |
| 37 | - |
45 min, complete inactivation | Clostridium paraputrificum |
| 50 | - |
10 min, 95% inactivation, 15 min complete inactivation | Clostridium paraputrificum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
oxidation of 12beta-hydroxy bile acids | Clostridium paraputrificum |
| 10 | - |
reduction of 12-oxo bile acids | Clostridium paraputrificum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | about half maximum activity | Clostridium paraputrificum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
most stable | Clostridium paraputrificum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Clostridium paraputrificum | |
| NADPH | specific for, no activity with NADH | Clostridium paraputrificum | |
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