Cloned (Comment) | Organism |
---|---|
gene TR2, enzyme overexpression via leaves transformation with Agrobacterium tumefaciens AGL0, quantitative PCR enzyme expression analysis | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme suppression in potato plants by RNA interference (RNAi) and enzyme overexpression. The overexpression and gene knockdown of StTRII alters tropinone reduction in vivo, and has great influence on calystegine accumulation, in contrast to genetic manipulation of isozyme St-TRI, EC 1.1.1.206, phenotypes, calystegine analysis, overview. A strong reduction of calystegines appears in the StTRII RNAi lines | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum tuberosum | tropinone feeding experiments | ? | - |
? | |
tropinone + NADPH + H+ | Solanum tuberosum | Solanum tuberosum tropinone reductase II reduces tropinone in vivo | pseudotropine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
cv. Desiree | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | tropinone feeding experiments | Solanum tuberosum | ? | - |
? | |
tropinone + NADPH + H+ | - |
Solanum tuberosum | pseudotropine + NADP+ | - |
r | |
tropinone + NADPH + H+ | Solanum tuberosum tropinone reductase II reduces tropinone in vivo | Solanum tuberosum | pseudotropine + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TR2 | - |
Solanum tuberosum |
TRII | - |
Solanum tuberosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Solanum tuberosum | |
NADPH | - |
Solanum tuberosum |
General Information | Comment | Organism |
---|---|---|
physiological function | pseudotropine forming tropinone reductase (TRII) catalyzes a tropinone reduction competing with TRI, EC 1.1.1.206. StTRII is the main enzyme catalyzing the synthesis of pseudotropine from tropinone. StTRII plays a role in calystegine formation in potato sprouts | Solanum tuberosum |