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Literature summary for 1.1.1.236 extracted from

  • Draeger, B.; Hashimoto, T.; Yamada, Y.
    Purification and characterization of pseudotropine forming tropinone reductase from Hyoscyamus niger root cultures (1988), Agric. Biol. Chem., 52, 2663-2667.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0211
-
NADPH
-
Hyoscyamus niger
0.0351
-
tropinone
-
Hyoscyamus niger
0.057
-
N-Methyl-4-piperidone
-
Hyoscyamus niger
0.299
-
N-propylyl-4-piperidone
-
Hyoscyamus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Hyoscyamus niger
84000
-
gel filtration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus niger
-
pseudotropine + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Datura stramonium
-
-
-
Hyoscyamus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Datura stramonium
-
root
-
Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.05
-
-
Hyoscyamus niger

Storage Stability

Storage Stability Organism
-20°C, enzyme preparation can be stored for 3 months without loss of activity Hyoscyamus niger
4°C, enzyme preparation can be stored for 4 days without loss of activity, when kept in 10% glycerol enzyme activity is lost within 2 days Hyoscyamus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-cyclohexanone + NADPH
-
Datura stramonium 4-methylcyclohexanol + NADP+
-
?
4-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 4-methylcyclohexanol + NADP+
-
?
4-piperidone + NADPH
-
Hyoscyamus niger piperidine-4-ol + NADP+
-
?
6-hydroxytropinone + NADPH
-
Datura stramonium ? + NADP+
-
?
6-hydroxytropinone + NADPH
-
Hyoscyamus niger ? + NADP+
-
?
N-methyl-4-piperidone + NADPH
-
Datura stramonium N-methylpiperidin-4-ol + NADP+
-
?
N-methyl-4-piperidone + NADPH more efficient substrate, 230% of activity with tropinone Hyoscyamus niger N-methylpiperidin-4-ol + NADP+
-
?
N-propyl-4-piperidone + NADPH
-
Datura stramonium N-propylpiperidin-4-ol + NADP+
-
?
N-propyl-4-piperidone + NADPH more efficient substrate, 300% of activity with tropinone Hyoscyamus niger N-propylpiperidin-4-ol + NADP+
-
?
nortropinone + NADPH
-
Hyoscyamus niger ? + NADP+
-
?
tetrahydrothiopyran-4-one + NADPH
-
Datura stramonium tetrahydrothiopyran-4-ol + NADP+
-
?
tetrahydrothiopyran-4-one + NADPH
-
Hyoscyamus niger tetrahydrothiopyran-4-ol + NADP+
-
?
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Datura stramonium
dimer 2 * 39000, SDS-PAGE Hyoscyamus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Hyoscyamus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24 56 77% of the maximum activity at 24°C, completely inactivated at 56°C Hyoscyamus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Hyoscyamus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 8.5 more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 Hyoscyamus niger

Cofactor

Cofactor Comment Organism Structure
NADH
-
Datura stramonium
NADH preference for NADPH, velocity 5.4% of the velocity found with NADPH Hyoscyamus niger
NADPH
-
Datura stramonium
NADPH
-
Hyoscyamus niger