KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0211 | - |
NADPH | - |
Hyoscyamus niger | |
0.0351 | - |
tropinone | - |
Hyoscyamus niger | |
0.057 | - |
N-Methyl-4-piperidone | - |
Hyoscyamus niger | |
0.299 | - |
N-propylyl-4-piperidone | - |
Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Hyoscyamus niger |
84000 | - |
gel filtration | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura stramonium | - |
- |
- |
Hyoscyamus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Datura stramonium | - |
root | - |
Hyoscyamus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
- |
Hyoscyamus niger |
Storage Stability | Organism |
---|---|
-20°C, enzyme preparation can be stored for 3 months without loss of activity | Hyoscyamus niger |
4°C, enzyme preparation can be stored for 4 days without loss of activity, when kept in 10% glycerol enzyme activity is lost within 2 days | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
4-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 4-methylcyclohexanol + NADP+ | - |
? | |
4-piperidone + NADPH | - |
Hyoscyamus niger | piperidine-4-ol + NADP+ | - |
? | |
6-hydroxytropinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
6-hydroxytropinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Datura stramonium | N-methylpiperidin-4-ol + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | more efficient substrate, 230% of activity with tropinone | Hyoscyamus niger | N-methylpiperidin-4-ol + NADP+ | - |
? | |
N-propyl-4-piperidone + NADPH | - |
Datura stramonium | N-propylpiperidin-4-ol + NADP+ | - |
? | |
N-propyl-4-piperidone + NADPH | more efficient substrate, 300% of activity with tropinone | Hyoscyamus niger | N-propylpiperidin-4-ol + NADP+ | - |
? | |
nortropinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
tetrahydrothiopyran-4-one + NADPH | - |
Datura stramonium | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
tetrahydrothiopyran-4-one + NADPH | - |
Hyoscyamus niger | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Datura stramonium |
dimer | 2 * 39000, SDS-PAGE | Hyoscyamus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Hyoscyamus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 56 | 77% of the maximum activity at 24°C, completely inactivated at 56°C | Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Hyoscyamus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 | Hyoscyamus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Datura stramonium | |
NADH | preference for NADPH, velocity 5.4% of the velocity found with NADPH | Hyoscyamus niger | |
NADPH | - |
Datura stramonium | |
NADPH | - |
Hyoscyamus niger |