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Literature summary for 1.1.1.231 extracted from

  • Korff, J.M.; Jarabak, J.
    The purification and characterization of a prostacyclin dehydrogenase from rabbit kidney (1982), J. Biol. Chem., 257, 2177-2181.
    View publication on PubMed
Search for more literature for 1.1.1.231

Inhibitors

Inhibitors Comment Organism Structure
Furosemide
-
 Oryctolagus cuniculus
NADPH
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 Phenanthrene quinone
-
 Oryctolagus cuniculus
0.00538
-
 NADP+
-
 Oryctolagus cuniculus
0.0667
-
 4-nitrobenzaldehyde
-
 Oryctolagus cuniculus
0.25
-
 menadione
-
 Oryctolagus cuniculus
0.364
-
 prostaglandin I2
-
 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, rabbit, SDS-PAGE Oryctolagus cuniculus
56000
-
 SDS-PAGE of amidinated enzyme Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ Oryctolagus cuniculus
-
 (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+ random mechanism or ordered mechanism with NADP+ binding first Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ i.e. prostaglandin I2, prostacyclin Oryctolagus cuniculus (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH the product is not stable and not available for analysis of the reverse reaction ?
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
-
 Oryctolagus cuniculus (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
 ?
4-nitrobenzaldehyde + NADP+ more rapid reaction than with prostacyclin Oryctolagus cuniculus 4-nitrobenzoate + NADPH
-
 ?
menadione + NADP+ more rapid reaction than with prostacyclin Oryctolagus cuniculus ?
-
 ?
phenanthrene quinone + NADP+ more rapid reaction than with prostacyclin Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, rabbit, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.3
-
 assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
additional information not a flavoprotein Oryctolagus cuniculus
NADP+
-
 Oryctolagus cuniculus