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Literature summary for 1.1.1.227 extracted from

  • Khine, A.; Yang, M.; Hu, A.; Lin, G.; Toh, Y.; Chen, H.
    Production of optically pure (-)-borneol by Pseudomonas monteilii TCU-CK1 and characterization of borneol dehydrogenase involved (2020), Enzyme Microb. Technol., 139, 109586 .
    View publication on PubMed
Search for more literature for 1.1.1.227

Inhibitors

Inhibitors Comment Organism Structure
(-)-Camphor
-
 Pseudomonas monteilii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 (-)-borneol pH 7, 25°C Pseudomonas monteilii
0.85
-
 (-)-borneol pH 11, 25°C Pseudomonas monteilii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-borneol + NAD+ Pseudomonas monteilii
-
 (-)-camphor + NADH + H+
-
 ?
additional information Pseudomonas monteilii (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 ?
-
-

Organism

Organism UniProt Comment Textmining
Pseudomonas monteilii
-
-
-
Pseudomonas monteilii TCU-CK1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns Pseudomonas monteilii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-borneol + NAD+
-
 Pseudomonas monteilii (-)-camphor + NADH + H+
-
 ?
additional information (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 Pseudomonas monteilii ?
-
-

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, x * 27400, calculated from sequence Pseudomonas monteilii

Synonyms

Synonyms Comment Organism
BDH cf. EC1.1.1.198 Pseudomonas monteilii
TCK1_3449
-
 Pseudomonas monteilii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.3
-
 (-)-borneol pH 7, 25°C Pseudomonas monteilii
29.4
-
 (-)-borneol pH 11, 25°C Pseudomonas monteilii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
12.5
-
-
 Pseudomonas monteilii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.03
-
 (-)-Camphor pH 7, 25°C Pseudomonas monteilii
1.07
-
 (-)-Camphor pH 11, 25°C Pseudomonas monteilii

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas monteilii calculated fromn sequence
-
 4.8

General Information

General Information Comment Organism
physiological function neither wild-tpe nor a gene deletion mutant is able to grow on (-)-borneol or (-)-camphor as sole carbon source. Both strains grow similarly on (+)-borneol or (+)-camphor Pseudomonas monteilii