Cloned (Comment) | Organism |
---|---|
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated | Phelipanche aegyptiaca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines | Phelipanche aegyptiaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phelipanche aegyptiaca | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root tubercle | - |
Phelipanche aegyptiaca | - |
shoot | underground | Phelipanche aegyptiaca | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannose 6-phosphate + NADPH + H+ | - |
Phelipanche aegyptiaca | D-mannitol 1-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M6PR | - |
Phelipanche aegyptiaca |
mannose 6-phosphate reductase | - |
Phelipanche aegyptiaca |