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Literature summary for 1.1.1.22 extracted from

  • Wei, S.; Kulinich, A.; Duan, X.C.; Liu, L.; Voglmeir, J.
    Discovery and biochemical characterization of UDP-glucose dehydrogenase from Granulibacter bethesdensis (2015), Protein Pept. Lett., 22, 628-634.
    View publication on PubMed

Application

Application Comment Organism
molecular biology the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems Granulibacter bethesdensis
synthesis the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems Granulibacter bethesdensis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme overexpression Granulibacter bethesdensis

Inhibitors

Inhibitors Comment Organism Structure
SDS strong inhibition Granulibacter bethesdensis
UDP-xylose allosteric feedback inhibitor, reduces significantly the activity of the enzyme Granulibacter bethesdensis
Urea strong inhibition Granulibacter bethesdensis
Zn2+ strong inhibition Granulibacter bethesdensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O Granulibacter bethesdensis
-
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates ?

Organism

Organism UniProt Comment Textmining
Granulibacter bethesdensis
-
originally isolated from the lymph nodes of patients with chronic granulomatous disease (CGD)
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, simple purification method Granulibacter bethesdensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
Granulibacter bethesdensis UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
?
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
Granulibacter bethesdensis UDP-alpha-D-glucuronate + 2 NADH + 2 H+ UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates ?

Synonyms

Synonyms Comment Organism
GbUGD
-
Granulibacter bethesdensis
UDP-glucose dehydrogenase
-
Granulibacter bethesdensis
UDPglucose dehydrogenase
-
Granulibacter bethesdensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
recombinant enzyme Granulibacter bethesdensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Granulibacter bethesdensis

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Granulibacter bethesdensis
NAD+ specific for Granulibacter bethesdensis