Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.22 extracted from

  • Egger, S.; Chaikuad, A.; Klimacek, M.; Kavanagh, K.L.; Oppermann, U.; Nidetzky, B.
    Structural and kinetic evidence that catalytic reaction of human UDP-glucose 6-dehydrogenase involves covalent thiohemiacetal and thioester enzyme intermediates (2012), J. Biol. Chem., 287, 2119-2129.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in mutant E161Q, the hydrolysis step becomes completely rate-limiting so that a thioester enzyme intermediate accumulates at steady state. Crystallization of mutant E161Q in the presence of 5 mM UDP-glucose and 2 mM NAD results in trapping a thiohemiacetal enzyme intermediate. Residue Cys276 is covalently modified in the structure, establishing its role as catalytic nucleophile of the reaction Homo sapiens

Protein Variants

Protein Variants Comment Organism
D280A extremely poor enzymic activity Homo sapiens
E161Q hydrolysis step becomes completely rate-limiting so that a thioester enzyme intermediate accumulates at steady state. Crystallization of E161Q in the presence of 5 mM UDP-glucose and 2 mM NAD results in trapping a thiohemiacetal enzyme intermediate Homo sapiens
K220A extremely poor enzymic activity Homo sapiens
N224A steady-state kinetic parameters are within an order of magnitude of the native enzyme Homo sapiens
T131S steady-state kinetic parameters are within an order of magnitude of the native enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 UDP-alpha-D-glucose wild-type, pH 8.7, 25°C Homo sapiens
0.055
-
 UDP-alpha-D-glucose mutant E161Q, pH 8.7, 25°C Homo sapiens
0.1
-
 NAD+ mutant E161Q, pH 8.7, 25°C Homo sapiens
0.7
-
 NAD+ wild-type, pH 8.7, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60701
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
 Homo sapiens UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
 UDP-alpha-D-glucose mutant E161Q, pH 8.7, 25°C Homo sapiens
0.85
-
 UDP-alpha-D-glucose wild-type, pH 8.7, 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
 NAD+ mutant E161Q, pH 8.7, 25°C Homo sapiens
0.025
-
 UDP-alpha-D-glucose mutant E161Q, pH 8.7, 25°C Homo sapiens
1.2
-
 NAD+ wild-type, pH 8.7, 25°C Homo sapiens
24
-
 UDP-alpha-D-glucose wild-type, pH 8.7, 25°C Homo sapiens