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Literature summary for 1.1.1.22 extracted from
- Characterization of human UDP-glucose dehydrogenase. CYS-276 is required for the second of two successive oxidations (2004), J. Biol. Chem., 279, 23590-23596.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C276S | no enzymic activity, affinity for NAD+ similar to wild-type, retains predominantly hexameric structure | Homo sapiens |
| K279A | no enzymic activity, affinity for NAD+ similar to wild-type, almost exclusively found as dimer | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
UDP-glucose | pH 7.4, 22°C | Homo sapiens |  |
| 0.355 | - |
NAD+ | pH 7.4, 22°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 345000 | - |
gel filtration, dynamic light scattering | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60701 | recombinant His-tagged enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 x 57000, SDS-PAGE | Homo sapiens |
| More | significant amount of dimeric and monomeric species can be detected | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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