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Literature summary for 1.1.1.22 extracted from

  • Campbell, R.E.; Sala, R.F.; van de Rijn, I.; Tanner, M.E.
    Properties and kinetic analysis of UDP-glucose dehydrogenase from group A streptococci (1997), J. Biol. Chem., 272, 3416-3422.
    View publication on PubMed
Search for more literature for 1.1.1.22

Application

Application Comment Organism
pharmacology target for inhibitor design Streptococcus pyogenes

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli JM109 Streptococcus pyogenes

General Stability

General Stability Organism
fairly stable with 2 mM dithiothreitol Streptococcus pyogenes
stable during repeated freezing and thawing cycles with 10% glycerol Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
thiol group modifying reagents
-
 Streptococcus pyogenes
UDP-D-xylose competitive with UDPglucose Streptococcus pyogenes
Uridine 5'-diphosphate chloroacetol alkylates a thiol group of cysteine in the catalytic centre via being specifically bound instead of the substrate Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 UDPglucose
-
 Streptococcus pyogenes
0.06
-
 NAD+
-
 Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 electrospray mass spectrometry Streptococcus pyogenes
52000
-
 gel filtation Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + NAD+ + H2O Streptococcus pyogenes delivers glucuronic acid for the formation of a antiphagocytic polysaccharide capsule that is required for virulence of pathogenic bacteria UDPglucuronate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
 group A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Streptococcus pyogenes

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + 2 NAD+ + H2O = UDP-alpha-D-glucuronate + 2 NADH + 2 H+ ping-pong reaction mechanism Streptococcus pyogenes
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
 recombinant and purified Streptococcus pyogenes

Storage Stability

Storage Stability Organism
5°C, 50 mM Tris-HCl, pH 8.7, 2 mM dithiothreitol, after 24 h 90% activity left Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
 Streptococcus pyogenes UDP-glucuronate + 2 NADH + 2 H+
-
 ?
UDPglucose + NAD+ + H2O delivers glucuronic acid for the formation of a antiphagocytic polysaccharide capsule that is required for virulence of pathogenic bacteria Streptococcus pyogenes UDPglucuronate + NADH
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45500, SDS-PAGE Streptococcus pyogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 after incubation for 1.6 h loss of 40% activity Streptococcus pyogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.117
-
 UDPglucose
-
 Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
additional information no constantly bound chromophoric cofactors, i.e. NAD+, required Streptococcus pyogenes