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Literature summary for 1.1.1.22 extracted from

  • Schiller, J.G.; Bowser, A.M.; Feingold, D.S.
    Partial purification and properties of UDPG dehydrogenase from Escherichia coli (1973), Biochim. Biophys. Acta, 293, 1-10.
    View publication on PubMed
Search for more literature for 1.1.1.22

Activating Compound

Activating Compound Comment Organism Structure
3-pyridinealdehyde adenine dinucleotide can replace NAD+ Escherichia coli
Deamino adenine dinucleotide can replace NAD+ Escherichia coli
Thionicotinamide adenine dinucleotide can replace NAD+ Escherichia coli

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Escherichia coli
UDPglucose stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NADH
-
 Escherichia coli
UDPglucuronate
-
 Escherichia coli
UDPxylose
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 NAD+
-
 Escherichia coli
1
-
 UDPglucose
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + NAD+ + H2O Escherichia coli
-
 UDPglucuronate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-4°C, frozen in presence of UDPglucose, stable for 2 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-glucose + NAD+ + H2O reaction rate is 5.5% of that with UDP-glucose Escherichia coli CDP-glucuronate + NADH
-
 ?
dTDP-glucose + NAD+ + H2O reaction rate is 16.7% of that with UDPglucose Escherichia coli dTDP-glucuronate + NADH
-
 ?
additional information cosubstrates which can replace NAD+: 3-acetylpyridine adenine dinucleotide Escherichia coli ?
-
 ?
additional information 3-pyridinealdehyde adenine dinucleotide Escherichia coli ?
-
 ?
additional information thionicotinamide adenine dinucleotide Escherichia coli ?
-
 ?
additional information no activity with GTP-glucose Escherichia coli ?
-
 ?
additional information no reaction with: 3-pyridinealdehyde deamino adenosine dinucleotide Escherichia coli ?
-
 ?
additional information deamino adenine dinucleotide Escherichia coli ?
-
 ?
additional information no activity with ADP-glucose Escherichia coli ?
-
 ?
TDP-glucose + NAD+ + H2O reaction rate is 17% of that with UDPglucose Escherichia coli TDP-glucuronate + NADH
-
 ?
UDP-glucose + 2 NAD+ + H2O
-
 Escherichia coli UDP-glucuronate + 2 NADH + 2 H+
-
 ?
UDP-glucose + 3-acetylpyridine adenine dinucleotide + H2O
-
 Escherichia coli UDP-glucuronate + ?
-
 ?
UDP-glucose + 3-pyridinealdehyde adenine dinucleotide
-
 Escherichia coli UDP-glucuronate
-
 ?
UDP-glucose + deamino adenine dinucleotide + H2O
-
 Escherichia coli UDP-glucuronate + ?
-
 ?
UDP-glucose + thionicotinamide adenine dinucleotide + H2O
-
 Escherichia coli UDP-glucuronate + ?
-
 ?
UDPglucose + NAD+ + H2O
-
 Escherichia coli UDPglucuronate + NADH
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.4 at pH 7.5 and 9.4 about 50% of activity maximum Escherichia coli

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine adenine dinucleotide can replace NAD+ Escherichia coli