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Literature summary for 1.1.1.219 extracted from

  • Ahmed, N.U.; Park, J.I.; Jung, H.J.; Yang, T.J.; Hur, Y.; Nou, I.S.
    Characterization of dihydroflavonol 4-reductase (DFR) genes and their association with cold and freezing stress in Brassica rapa (2014), Gene, 550, 46-55.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene BrDFR1, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR10, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR11, on chromosome A03, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR12, on chromosome A08, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR2, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR3, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR3, on chromosome A02, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR4, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR5, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR6, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR7, on chromosome A09, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR8, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis
gene BrDFR9, on chromosome A06, genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, RT-PCR expression profiling of genotypes, and real-time quantitative PCR analysis Brassica rapa subsp. pekinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34762
-
-
Brassica rapa subsp. pekinensis
34860
-
-
Brassica rapa subsp. pekinensis
35420
-
-
Brassica rapa subsp. pekinensis
35520
-
-
Brassica rapa subsp. pekinensis
35860
-
-
Brassica rapa subsp. pekinensis
35910
-
-
Brassica rapa subsp. pekinensis
36158
-
-
Brassica rapa subsp. pekinensis
36320
-
-
Brassica rapa subsp. pekinensis
39650
-
-
Brassica rapa subsp. pekinensis
40450
-
-
Brassica rapa subsp. pekinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrokaempferol + NADPH + H+ Brassica rapa subsp. pekinensis
-
leucopelargonidin + NADP+
-
?
dihydromyricetin + NADPH + H+ Brassica rapa subsp. pekinensis
-
leucodelphinidin + NADP+
-
?
dihydroquercetin + NADPH + H+ Brassica rapa subsp. pekinensis
-
cis-3,4-leucocyanidin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. pekinensis M4D235 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4DRB9 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4DU26 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4DU27 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4DU28 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4E380 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4E7I9 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4E7J1 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4E7J2 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4ECU0 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4ES88 cvs. Palgangbaechu, 8053, and Seoulbaechu
-
Brassica rapa subsp. pekinensis M4FB76 cvs. Palgangbaechu, 8053, and Seoulbaechu
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
Brassica rapa subsp. pekinensis
-
flower bud high expression level Brassica rapa subsp. pekinensis
-
leaf
-
Brassica rapa subsp. pekinensis
-
additional information organ-specific DFR expression analysis Brassica rapa subsp. pekinensis
-
root
-
Brassica rapa subsp. pekinensis
-
stem
-
Brassica rapa subsp. pekinensis
-
stem weak expression Brassica rapa subsp. pekinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + NADPH + H+
-
Brassica rapa subsp. pekinensis leucopelargonidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Brassica rapa subsp. pekinensis leucodelphinidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Brassica rapa subsp. pekinensis cis-3,4-leucocyanidin + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 34760, sequence calculation Brassica rapa subsp. pekinensis
? x * 34860, sequence calculation Brassica rapa subsp. pekinensis
? x * 35420, sequence calculation Brassica rapa subsp. pekinensis
? x * 35520, sequence calculation Brassica rapa subsp. pekinensis
? x * 35860, sequence calculation Brassica rapa subsp. pekinensis
? x * 35910, sequence calculation Brassica rapa subsp. pekinensis
? x * 36150, sequence calculation Brassica rapa subsp. pekinensis
? x * 36320, sequence calculation Brassica rapa subsp. pekinensis
? x * 39650, sequence calculation Brassica rapa subsp. pekinensis
? x * 40450, sequence calculation Brassica rapa subsp. pekinensis
More sequence analysis and structure homology, overview Brassica rapa subsp. pekinensis

Synonyms

Synonyms Comment Organism
BrDFR1
-
Brassica rapa subsp. pekinensis
BrDFR10
-
Brassica rapa subsp. pekinensis
BrDFR11
-
Brassica rapa subsp. pekinensis
BrDFR12
-
Brassica rapa subsp. pekinensis
BrDFR2
-
Brassica rapa subsp. pekinensis
BrDFR3
-
Brassica rapa subsp. pekinensis
BrDFR4
-
Brassica rapa subsp. pekinensis
BrDFR5
-
Brassica rapa subsp. pekinensis
BrDFR6
-
Brassica rapa subsp. pekinensis
BrDFR7
-
Brassica rapa subsp. pekinensis
BrDFR8
-
Brassica rapa subsp. pekinensis
BrDFR9
-
Brassica rapa subsp. pekinensis
DFR
-
Brassica rapa subsp. pekinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Brassica rapa subsp. pekinensis

pI Value

Organism Comment pI Value Maximum pI Value
Brassica rapa subsp. pekinensis sequence calculation
-
5.2
Brassica rapa subsp. pekinensis sequence calculation
-
5.21
Brassica rapa subsp. pekinensis sequence calculation
-
5.26
Brassica rapa subsp. pekinensis sequence calculation
-
5.36
Brassica rapa subsp. pekinensis sequence calculation
-
5.41
Brassica rapa subsp. pekinensis sequence calculation
-
5.45
Brassica rapa subsp. pekinensis sequence calculation
-
5.49
Brassica rapa subsp. pekinensis sequence calculation
-
5.66
Brassica rapa subsp. pekinensis sequence calculation
-
5.9
Brassica rapa subsp. pekinensis sequence calculation
-
6.09
Brassica rapa subsp. pekinensis sequence calculation
-
6.2
Brassica rapa subsp. pekinensis sequence calculation
-
8.65

Expression

Organism Comment Expression
Brassica rapa subsp. pekinensis induction of gene BrDFR10 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR2 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR3 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR4 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR5 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR6 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR8 by cold stress dependent on the genotypes, overview up
Brassica rapa subsp. pekinensis induction of gene BrDFR9 by cold stress dependent on the genotypes, overview up

General Information

General Information Comment Organism
physiological function BrDFR isozymes are regulated by two transcription factors, BrMYB2-2 and BrTT8, contrasting with anthocyanin accumulation and cold and freezing stress Brassica rapa subsp. pekinensis