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Literature summary for 1.1.1.217 extracted from

  • Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.
    Purification and properties of an asymmetric reduction enzyme of 2-methyl-3-oxobutyrate in baker's yeast (1985), Agric. Biol. Chem., 49, 2563-2570.
No PubMed abstract available
Search for more literature for 1.1.1.217

Inhibitors

Inhibitors Comment Organism Structure
FMN
-
 Saccharomyces cerevisiae
KCN
-
 Saccharomyces cerevisiae
NADH
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 Benzyl 2-methyl-3-oxobutyrate
-
 Saccharomyces cerevisiae
4.2
-
 benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
-
 Saccharomyces cerevisiae
4.2
-
 benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ strong inhibition Saccharomyces cerevisiae
Cu2+ strong inhibition Saccharomyces cerevisiae
Hg2+ strong inhibition Saccharomyces cerevisiae
Ni2+ strong inhibition Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Saccharomyces cerevisiae
32000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+ Saccharomyces cerevisiae
-
 benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0063
-
 crude extract Saccharomyces cerevisiae
0.9
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
 Saccharomyces cerevisiae benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
-
 r
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
-
 Saccharomyces cerevisiae benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+ ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme r
additional information no reduction of aldehydes or alcohols Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 rapid loss of activity above 40°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 8 less than 10% activity at pH 4 and 9 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Saccharomyces cerevisiae