Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Saccharomyces cerevisiae | |
KCN | - |
Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
Benzyl 2-methyl-3-oxobutyrate | - |
Saccharomyces cerevisiae | |
4.2 | - |
benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate | - |
Saccharomyces cerevisiae | |
4.2 | - |
benzyl(2S,3S)-2-methyl-3-hydroxybutanoate | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Saccharomyces cerevisiae | |
Cu2+ | strong inhibition | Saccharomyces cerevisiae | |
Hg2+ | strong inhibition | Saccharomyces cerevisiae | |
Ni2+ | strong inhibition | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
32000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+ | Saccharomyces cerevisiae | - |
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0063 | - |
crude extract | Saccharomyces cerevisiae |
0.9 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+ | - |
Saccharomyces cerevisiae | benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+ | - |
r | |
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+ | - |
Saccharomyces cerevisiae | benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+ | ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme | r | |
additional information | no reduction of aldehydes or alcohols | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
rapid loss of activity above 40°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | less than 10% activity at pH 4 and 9 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae |