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Literature summary for 1.1.1.215 extracted from

  • Li, K.; Mao, X.; Liu, L.; Lin, J.; Sun, M.; Wei, D.; Yang, S.
    Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-D-gluconic acid by Gluconobacter oxydans (2016), Microb. Cell Fact., 15, 121.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.215

Application

Application Comment Organism
synthesis ga2dh-overexpressing strain Gluconobacter oxydans_tufB_ga2dh is a useful species for use in 2-dehydro-D-gluconate production Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
gene ga2dh, recombinant overexpression of the enzyme in Gluconobacter oxydans strain DSM2003 under the control of three promoters, PtufB, Pga2dh or Pghp0169, respectively. Recombinant strain Gluconobacter oxydans_tufB_ga2dh shows a similar growth rate to that of the control strain and displays the highest specific productivity of 2-dehydro-D-gluconate from D-gluconate, which is increased nearly twofold compared with that of the control strain during batch biotransformation, method optimization Gluconobacter oxydans

Protein Variants

Protein Variants Comment Organism
additional information development of an efficient bacterial strain of Gluconobacter oxydans_tufB_ga2dh for the production of 2-dehydro-D-gluconate by overexpressing the ga2dh gene in Gluconobacter oxydans. Supply of sufficient oxygen enhances the positive effect of gene overexpression on 2-dehydro-D-gluconate production. Gluconobacter oxydans_tufB_ga2dh is a competitive species for use in 2-dehydro-D-gluconate production. Transgenic strains, evaluation and optimization, detailed overview Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Gluconobacter oxydans 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate + NADP+ Gluconobacter oxydans
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?
D-gluconate + NADP+ Gluconobacter oxydans 621H
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?
additional information Gluconobacter oxydans for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate ?
-
 ?
additional information Gluconobacter oxydans 621H for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q5FRK5
-
-
Gluconobacter oxydans 621H Q5FRK5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + NADP+
-
 Gluconobacter oxydans 2-dehydro-D-gluconate + NADPH + H+
-
 ?
D-gluconate + NADP+
-
 Gluconobacter oxydans 621H 2-dehydro-D-gluconate + NADPH + H+
-
 ?
additional information for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate Gluconobacter oxydans ?
-
 ?
additional information for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate Gluconobacter oxydans 621H ?
-
 ?

Synonyms

Synonyms Comment Organism
GA2DH
-
 Gluconobacter oxydans
gluconate-2-dehydrogenase
-
 Gluconobacter oxydans
GOX1230
-
 Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Gluconobacter oxydans