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Literature summary for 1.1.1.215 extracted from

  • Yum, D-Y.; Lee, B-Y.; Hahm, D-H.; Pan, J-G.
    The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase (1998), J. Bacteriol., 180, 5984-5988.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.215

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Escherichia coli
74000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-diketo-D-gluconate + NADPH Escherichia coli
-
 5-keto-D-gluconate + NADP+
-
 ?
2-dehydro-D-gluconate + NADPH Escherichia coli
-
 D-gluconate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 constitutive expressed enzyme
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged enzyme, metal-chelate affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diketo-D-gluconate + NADPH
-
 Escherichia coli 5-keto-D-gluconate + NADP+
-
 ?
2-dehydro-D-gluconate + NADPH
-
 Escherichia coli D-gluconate + NADP+
-
 ?
2-keto-L-gulonate + NADPH
-
 Escherichia coli L-idonate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Escherichia coli