KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | - |
Brevibacterium ketosoreductum | |
5 | - |
2,5-diketo-D-gluconate | - |
Brevibacterium ketosoreductum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Brevibacterium ketosoreductum |
72000 | - |
gel filtration | Brevibacterium ketosoreductum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diketo-D-gluconate + NADPH | Brevibacterium ketosoreductum | - |
5-keto-D-gluconate + NADP+ | - |
ir | |
2,5-diketo-D-gluconate + NADPH | Brevibacterium ketosoreductum ATCC 21914 | - |
5-keto-D-gluconate + NADP+ | - |
ir | |
2-dehydro-D-gluconate + NADPH | Brevibacterium ketosoreductum | - |
D-gluconate + NADP+ | - |
? | |
2-dehydro-D-gluconate + NADPH | Brevibacterium ketosoreductum ATCC 21914 | - |
D-gluconate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium ketosoreductum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200 | Brevibacterium ketosoreductum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.2 | - |
- |
Brevibacterium ketosoreductum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diketo-D-gluconate + NADPH | - |
Brevibacterium ketosoreductum | 5-keto-D-gluconate + NADP+ | - |
ir | |
2,5-diketo-D-gluconate + NADPH | - |
Brevibacterium ketosoreductum ATCC 21914 | 5-keto-D-gluconate + NADP+ | - |
ir | |
2-dehydro-D-gluconate + NADPH | - |
Brevibacterium ketosoreductum | D-gluconate + NADP+ | - |
? | |
2-dehydro-D-gluconate + NADPH | 11.8% of activity with 2,5-diketo-D-gluconate | Brevibacterium ketosoreductum | D-gluconate + NADP+ | - |
? | |
2-dehydro-D-gluconate + NADPH | - |
Brevibacterium ketosoreductum ATCC 21914 | D-gluconate + NADP+ | - |
? | |
2-dehydro-D-gluconate + NADPH | 11.8% of activity with 2,5-diketo-D-gluconate | Brevibacterium ketosoreductum ATCC 21914 | D-gluconate + NADP+ | - |
? | |
2-keto-L-gulonate + NADPH | 10.8% of activity with 2,5-diketo-D-gluconate | Brevibacterium ketosoreductum | L-idonate + NADP+ | - |
? | |
2-keto-L-gulonate + NADPH | 10.8% of activity with 2,5-diketo-D-gluconate | Brevibacterium ketosoreductum ATCC 21914 | L-idonate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Brevibacterium ketosoreductum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Brevibacterium ketosoreductum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 35% of activity left after incubation at pH 8.4 for 2 h at 30°C, no remaining activity below pH 5.2 or above pH 10.0 | Brevibacterium ketosoreductum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Brevibacterium ketosoreductum | |
NADPH | - |
Brevibacterium ketosoreductum |