Crystallization (Comment) | Organism |
---|---|
- |
Acetobacter ascendens |
General Stability | Organism |
---|---|
2-ketogluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l | Acetobacter ascendens |
5-ketogluconate does not stabilize | Acetobacter ascendens |
gluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l | Acetobacter ascendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition | Acetobacter ascendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
NADPH | - |
Acetobacter ascendens | |
1.2 | - |
NADP+ | - |
Acetobacter ascendens | |
4.4 | - |
gluconate | - |
Acetobacter ascendens | |
5.3 | - |
2-keto-D-gluconate | - |
Acetobacter ascendens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Acetobacter ascendens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal requirement | Acetobacter ascendens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
3 * 40000, SDS-PAGE | Acetobacter ascendens |
120000 | - |
gel filtration | Acetobacter ascendens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter ascendens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on Blue-Dextran Sepharose B | Acetobacter ascendens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
187 | - |
- |
Acetobacter ascendens |
Storage Stability | Organism |
---|---|
ammonium sulfate solution of crystalline enzyme stable for over 2 years | Acetobacter ascendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + NADPH | i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter | Acetobacter ascendens | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | equal reaction rate of reduction of 2-ketogluconate and oxidation of gluconate with Gluconobacter enzyme | Acetobacter ascendens | D-gluconate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | - |
Acetobacter ascendens | L-idonate + NADP+ | - |
r | |
5-keto-D-gluconate + NADPH | poor substrate | Acetobacter ascendens | D-gluconate + NADP+ | - |
? | |
D-galactonate + NADP+ | - |
Acetobacter ascendens | 2-dehydro-D-galactonate + NADPH | - |
r | |
L-idonate + NADP+ | - |
Acetobacter ascendens | 2-keto-L-idonate + NADPH | - |
? | |
additional information | D-xylonate, D-glucose, D-fructose, L-sorbose, 5-keto-D-fructose, D-sorbitol, glycerol | Acetobacter ascendens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 40000, SDS-PAGE | Acetobacter ascendens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Acetobacter ascendens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 65 | about half-maximal activity at 35°C and 65°C | Acetobacter ascendens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
stable for 10 min, oxidation activity | Acetobacter ascendens |
53 | - |
stable for 10 min, reduction activity | Acetobacter ascendens |
70 | - |
stable for 10 min in the presence of either 2-ketogluconate or gluconate | Acetobacter ascendens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of 2-ketogluconate | Acetobacter ascendens |
10.5 | - |
- |
Acetobacter ascendens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate | Acetobacter ascendens |
10 | 12 | about half-maximal activity at pH 10 and 12, oxidation of gluconate | Acetobacter ascendens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Acetobacter ascendens | |
NADPH | - |
Acetobacter ascendens |