Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain C41(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | transient single and multiple turnover kinetics, stopped flow kinetics, isotope kinetics, ligand binding analysis, kinetics with deuterium-substituted cofactor and binary complex of enzyme and cofactor, overview | Homo sapiens | |
0.0029 | - |
5alpha-dihydrotestosterone | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
0.0031 | - |
3alpha-androstanediol | pH 7.0, 25°C, recombinant enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH + H+ | Homo sapiens | inactivation of the potent androgen in human prostate | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain C41(DE3) in a successive chromatographic procedure, to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-acenaphthenol + NADPH | - |
Homo sapiens | ? | - |
? | |
5alpha-dihydrotestosterone + NADPH + H+ | inactivation of the potent androgen in human prostate | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
r | |
5alpha-dihydrotestosterone + NADPH + H+ | i.e. DHT | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AKR1C2 | - |
Homo sapiens |
type 3 3alpha-hydroxysteroid dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
3alpha-androstanediol | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
0.033 | - |
5alpha-dihydrotestosterone | pH 7.0, 25°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | comparison of NADPH with deuterium-substituted NADPD, overview | Homo sapiens |