Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Mentha x piperita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
NADP+ | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
0.01 | - |
NADPH | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
0.674 | - |
(-)-menthone | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
1 | - |
(+)-isomenthone | above, pH 9.3, 31°C, recombinant enzyme | Mentha x piperita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35722 | - |
x * 35722, amino acid sequence calculation | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-isomenthone + NADPH | Mentha x piperita | low activity | (+)-isomenthol + NADP+ | - |
? | |
(-)-menthone + NADPH + H+ | Mentha x piperita | - |
(3S)-neomenthol + NADP+ | - |
r | |
additional information | Mentha x piperita | primary monoterpene biosynthesis pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | - |
cv. Black Mitchum | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially | Mentha x piperita |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mentha x piperita | - |
oil gland secretory cell | - |
Mentha x piperita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-isomenthone + NADPH | low activity | Mentha x piperita | (+)-isomenthol + NADP+ | - |
? | |
(-)-menthone + NADPH + H+ | - |
Mentha x piperita | (3S)-neomenthol + NADP+ | - |
r | |
(-)-menthone + NADPH + H+ | low activity in the reverse reaction | Mentha x piperita | (3S)-neomenthol + NADP+ | (3S)-neomenthol is the main product, product determination by gas chromatography, overview | r | |
additional information | primary monoterpene biosynthesis pathway, overview | Mentha x piperita | ? | - |
? | |
additional information | monoterpenol is a poor substrate | Mentha x piperita | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35722, amino acid sequence calculation | Mentha x piperita |
Synonyms | Comment | Organism |
---|---|---|
(-)-menthone:(3S)-neomenthol reductase | - |
Mentha x piperita |
MNR | - |
Mentha x piperita |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
assay at | Mentha x piperita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NADPH | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita | |
0.06 | - |
(-)-menthone | pH 9.3, 31°C, recombinant enzyme | Mentha x piperita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Mentha x piperita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | 50% of maximal activity at pH 6.5 and pH 10.5 | Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mentha x piperita | |
NADPH | - |
Mentha x piperita |