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Literature summary for 1.1.1.206 extracted from
- Protein structure modeling indicates hexahistidine-tag interference with enzyme activity (2008), Proteins, 72, 173-183.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene trI, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Solanum dulcamara |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | Solanum dulcamara | the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview | tropine + NADP+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum dulcamara | - |
gene trI | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Solanum dulcamara |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | - |
Solanum dulcamara | tropine + NADP+ | - |
r | |
| tropinone + NADPH + H+ | the enzyme is involved in tropane alkaloid biosynthesis, e.g. of (S)-hyoscyamine, overview | Solanum dulcamara | tropine + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the family of short chain dehydrogenases | Solanum dulcamara |
| TRI | - |
Solanum dulcamara |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Solanum dulcamara | |
| NADPH | - |
Solanum dulcamara | |
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