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Literature summary for 1.1.1.206 extracted from
- Partial purification and properties of tropine dehydrogenase from root cultures of Datura stramonium (1982), J. Med. Plant Res., 44, 227-230.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | 14% inhibition at 0.01 mM, 56% inhibition at 0.1 mM, 100% inhibition at 1.0 mM | Datura stramonium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADP+ | - |
Datura stramonium | |
| 0.023 | - |
NADPH | - |
Datura stramonium | |
| 0.77 | - |
Tropine | - |
Datura stramonium | |
| 0.83 | - |
tropinone | - |
Datura stramonium | |
| 0.91 | - |
Scopine | - |
Datura stramonium | |
| 2 | - |
nortropine | - |
Datura stramonium | |
| 3.03 | - |
Nortropinone | - |
Datura stramonium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Datura stramonium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropine + NADP+ | Datura stramonium | last step in biosynthesis of tropine | tropinone + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Datura stramonium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Datura stramonium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Datura stramonium | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 50% glycerol, 3 weeks | Datura stramonium |
| 4°C, 0.46 mg protein/ml, complete inactivation of enzyme activity over night | Datura stramonium |
| 4°C, 2.3 mg protein/ml, 2 days, complete inactivation of enzyme activity | Datura stramonium |
| 4°C, 23 mg protein/ml, 9 days, complete inactivation of enzyme activity | Datura stramonium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nortropine + NADP+ | - |
Datura stramonium | nortropinone + NADPH | - |
? | |
| scopine + NADP+ | - |
Datura stramonium | scopinone + NADPH | - |
? | |
| tropine + NADP+ | - |
Datura stramonium | tropinone + NADPH | - |
r | |
| tropine + NADP+ | last step in biosynthesis of tropine | Datura stramonium | tropinone + NADPH | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Datura stramonium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Datura stramonium |
| 60 | - |
thermal denaturation within 10 min | Datura stramonium |
| 70 | - |
thermal denaturation within 3.5 min | Datura stramonium |
| 75 | - |
immediate thermal denaturation | Datura stramonium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
tropinone reduction | Datura stramonium |
| 9.5 | - |
tropine oxidation | Datura stramonium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | - |
Datura stramonium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Datura stramonium | |
| NADPH | - |
Datura stramonium | |
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Release 2023.1 (January 2023)
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