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Literature summary for 1.1.1.205 extracted from
- Oxanosine monophosphate is a covalent inhibitor of inosine 5'-monophosphate dehydrogenase (2019), Chem. Res. Toxicol., 32, 456-466 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Cryptosporidium parvum |
| expressed in Escherichia coli BL21 cells | Homo sapiens |
| expressed in Escherichia coli BL21 cells | Tritrichomonas foetus |
| expressed in Escherichia coli BL21 cells | Bacillus anthracis |
| expressed in Escherichia coli BL21 cells | Campylobacter jejuni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme in complex with oxanosine monophosphate, sitting drop vapor diffusion method, using 0.1 M lithium sulfate, 0.1 MES, pH 6.0, and 35% (v/v) 2-methyl-2,4-pentanediol | Campylobacter jejuni |
| enzyme in complex with oxanosine monophosphate, sitting drop vapor diffusion method, using 0.1 M magnesium chloride, 0.1 M MES, pH 6.5, and 30% (v/v) PEG 400 | Bacillus anthracis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GMP | - |
Bacillus anthracis |  |
| GMP | - |
Campylobacter jejuni | |
| GMP | - |
Cryptosporidium parvum | |
| GMP | - |
Homo sapiens | |
| GMP | - |
Tritrichomonas foetus | |
| oxanosine monophosphate | potent reversible competitive inhibitor | Bacillus anthracis | |
| oxanosine monophosphate | potent reversible competitive inhibitor | Campylobacter jejuni | |
| oxanosine monophosphate | potent reversible competitive inhibitor | Cryptosporidium parvum | |
| oxanosine monophosphate | potent reversible competitive inhibitor | Homo sapiens | |
| oxanosine monophosphate | potent reversible competitive inhibitor | Tritrichomonas foetus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
IMP | at pH 8.0 and 25°C | Homo sapiens | |
| 0.0063 | - |
IMP | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 0.007 | - |
NAD+ | at pH 8.0 and 25°C | Homo sapiens | |
| 0.01 | - |
IMP | at pH 8.0 and 25°C | Cryptosporidium parvum | |
| 0.012 | - |
IMP | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 0.045 | - |
NAD+ | at pH 8.0 and 25°C | Cryptosporidium parvum | |
| 0.066 | - |
NAD+ | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 0.08 | - |
NAD+ | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 0.083 | - |
IMP | at pH 8.0 and 25°C | Bacillus anthracis | |
| 0.7 | - |
NAD+ | at pH 8.0 and 25°C | Bacillus anthracis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IMP + NAD+ + H2O | Cryptosporidium parvum | - |
XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | Homo sapiens | - |
XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | Tritrichomonas foetus | - |
XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | Bacillus anthracis | - |
XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | Campylobacter jejuni | - |
XMP + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | A0A6L8P2U9 | - |
- |
| Campylobacter jejuni | A0A2R4D3F6 | - |
- |
| Cryptosporidium parvum | - |
- |
- |
| Homo sapiens | P12268 | - |
- |
| Tritrichomonas foetus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration | Cryptosporidium parvum |
| Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration | Homo sapiens |
| Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration | Tritrichomonas foetus |
| Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration | Bacillus anthracis |
| Ni-NTA Sepharose bead chromatography and Superdex 200 gel filtration | Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| IMP + NAD+ + H2O | - |
Cryptosporidium parvum | XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | - |
Homo sapiens | XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | - |
Tritrichomonas foetus | XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | - |
Bacillus anthracis | XMP + NADH + H+ | - |
? | |
| IMP + NAD+ + H2O | - |
Campylobacter jejuni | XMP + NADH + H+ | - |
? | |
| additional information | the enzyme does not hydrolyze oxanosine monophosphate | Cryptosporidium parvum | ? | - |
- |
|
| additional information | the enzyme does not hydrolyze oxanosine monophosphate | Homo sapiens | ? | - |
- |
|
| additional information | the enzyme does not hydrolyze oxanosine monophosphate | Tritrichomonas foetus | ? | - |
- |
|
| additional information | the enzyme does not hydrolyze oxanosine monophosphate | Bacillus anthracis | ? | - |
- |
|
| additional information | the enzyme does not hydrolyze oxanosine monophosphate | Campylobacter jejuni | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMPDH | - |
Cryptosporidium parvum |
| IMPDH | - |
Tritrichomonas foetus |
| IMPDH | - |
Bacillus anthracis |
| IMPDH | - |
Campylobacter jejuni |
| IMPDH2 | isoform | Homo sapiens |
| inosine 5'-monophosphate dehydrogenase | - |
Cryptosporidium parvum |
| inosine 5'-monophosphate dehydrogenase | - |
Homo sapiens |
| inosine 5'-monophosphate dehydrogenase | - |
Tritrichomonas foetus |
| inosine 5'-monophosphate dehydrogenase | - |
Bacillus anthracis |
| inosine 5'-monophosphate dehydrogenase | - |
Campylobacter jejuni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
NAD+ | at pH 8.0 and 25°C | Homo sapiens | |
| 0.22 | - |
IMP | at pH 8.0 and 25°C | Homo sapiens | |
| 0.7 | - |
NAD+ | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 0.7 | - |
IMP | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 2.2 | - |
NAD+ | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 2.2 | - |
IMP | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 3 | - |
NAD+ | at pH 8.0 and 25°C | Bacillus anthracis | |
| 3 | - |
IMP | at pH 8.0 and 25°C | Bacillus anthracis | |
| 3.2 | - |
NAD+ | at pH 8.0 and 25°C | Cryptosporidium parvum | |
| 3.2 | - |
IMP | at pH 8.0 and 25°C | Cryptosporidium parvum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Cryptosporidium parvum | |
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Tritrichomonas foetus | |
| NAD+ | - |
Bacillus anthracis | |
| NAD+ | - |
Campylobacter jejuni | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000013 | - |
oxanosine monophosphate | at pH 8.0 and 25°C | Homo sapiens | |
| 0.000017 | - |
oxanosine monophosphate | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 0.000051 | - |
oxanosine monophosphate | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 0.00009 | - |
oxanosine monophosphate | at pH 8.0 and 25°C | Cryptosporidium parvum | |
| 0.00034 | - |
oxanosine monophosphate | at pH 8.0 and 25°C | Bacillus anthracis | |
| 0.008 | - |
GMP | at pH 8.0 and 25°C | Campylobacter jejuni | |
| 0.021 | - |
GMP | at pH 8.0 and 25°C | Cryptosporidium parvum | |
| 0.024 | - |
GMP | at pH 8.0 and 25°C | Tritrichomonas foetus | |
| 0.037 | - |
GMP | at pH 8.0 and 25°C | Homo sapiens | |
| 0.115 | - |
GMP | at pH 8.0 and 25°C | Bacillus anthracis | |
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Release 2023.1 (January 2023)
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