Application | Comment | Organism |
---|---|---|
medicine | MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents | Clostridium perfringens |
medicine | MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents | Vibrio cholerae serotype O1 |
medicine | MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents | Campylobacter jejuni |
medicine | MPDH inhibitors are used as immunosuppressive, antiviral, and anticancer agents | Bacillus anthracis |
Cloned (Comment) | Organism |
---|---|
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long and short enzyme CBS-deletion mutants, BaIMPDHDELTAL and BaIMPDHDELTAS, in Escherichia coli | Bacillus anthracis |
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long enzyme CBS-deletion mutant, ClpIMPDHDELTAL, in Escherichia coli | Clostridium perfringens |
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged long enzyme CBS-deletion mutant, VcIMPDHDELTAL, in Escherichia coli | Vibrio cholerae serotype O1 |
gene impdh, sequence comparisons, recombinant expression of N-terminally His-tagged short enzyme CBS-deletion mutant, CjIMPDHDELTAS, in Escherichia coli | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
purified recombinant long enzyme mutant ClpIMPDHDELTAL in complex with IMP and with inhibitor 1 from 5% tacsimate, pH 7.0, 0.1 M HEPES, pH 7.0, 10% PEG MME 5000, 16°C, or with inhibitor 2 from 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG 10000, 16°C, X-ray diffraction structure determination and analysis at resolutions of 2.95 and 2.85 A, respectively | Clostridium perfringens |
purified recombinant long enzyme mutant VcIMPDHDELTAL in complex with IMP and NAD+ from 0.77 M sodium/potassium phosphate, 0.15 M Tris-HCl, pH 8.0, 6% MPD, 16°C, followed by soaking with 200 mM NAD+ solution for 15 min at 20°C, or enzyme mutant VcIMPDHDELTAL in complex with XMP and NADH from 1.03 M sodium/potassium phosphate, pH 5.0, 0.15 M sodium malate, 3% PEG 300, 16°C, followed by soaking with 200 mM NAD+ solution for 5 days at 16°C, X-ray diffraction structure determination and analysis at resolutions of 2.38 and 1.66 A, respectively | Vibrio cholerae serotype O1 |
purified recombinant short enzyme mutant BaIMPDHDELTAS in apoform from 0.2 M sodium chloride, 0.1 M sodium cacodylate, pH 6.5, and 2 M ammonium sulfate, 16°C, and purified recombinant long enzyme mutant BaIMPDHDELTAL in complex with IMP and with inhibitors 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide, ((alpha-methyl-)N-2-naphthalenyl-2-(2-pyridinyl)-1H-benzimidazole-)1-acetamide, 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide, 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide, N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea, and 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide in several conformations and under different conditions resulting in 6 different crystal structures, X-ray diffraction structure determination and analysis at resolutions of 1.93-2.85 A, respectively, overview | Bacillus anthracis |
purified recombinant short enzyme mutant CjIMPDHDELTAS in complex with IMP and with inhibitor 2 from 1.6 M ammonium sulfate, 0.1 M MES, pH 6.5, 10% dioxane, 16 °C, or with inhibitor 4 from 0.2 M lithium sulfate, 0.1 M CAPS, pH 10.5, 1.2 M sodium/0.8 M potassium phosphate, 16 °C, X-ray diffraction structure determination and analysis at resolutions of 2.44 and 2.54 A, respectively | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a long CBS domain deletion mutant variant, ClpIMPDHDELTAL. Deletion of residues Gln89-Arg215 | Clostridium perfringens |
additional information | construction of a long CBS domain deletion mutant variant, VcIMPDHDELTAL. Deletion of residues Phe91-Arg219 | Vibrio cholerae serotype O1 |
additional information | construction of a short and a long CBS domain deletion mutant variant, BaIMPDHDELTAS and BaIMPDHDELTAL. Deletion of residues Val95-Thr200 and Glu92-Arg220, respectively | Bacillus anthracis |
additional information | construction of a short CBS domain deletion mutant variant, CjIMPDHDELTAS. Deletion of residues Val92-Thr195 | Campylobacter jejuni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | - |
Bacillus anthracis | |
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | - |
Campylobacter jejuni | |
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | - |
Clostridium perfringens | |
2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | - |
Vibrio cholerae serotype O1 | |
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | - |
Bacillus anthracis | |
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | - |
Campylobacter jejuni | |
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | - |
Clostridium perfringens | |
2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | - |
Vibrio cholerae serotype O1 | |
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | - |
Bacillus anthracis | |
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | - |
Campylobacter jejuni | |
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | - |
Clostridium perfringens | |
3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | - |
Vibrio cholerae serotype O1 | |
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | - |
Bacillus anthracis | |
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | - |
Campylobacter jejuni | |
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | - |
Clostridium perfringens | |
4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | - |
Vibrio cholerae serotype O1 | |
additional information | inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variants, overview | Bacillus anthracis | |
additional information | inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variant, overview | Campylobacter jejuni | |
additional information | inhibitor synthesis and binding structure to the enzyme determined with the CBS deletion mutant enzyme variant, overview | Clostridium perfringens | |
additional information | inhibitor synthesis, overview | Vibrio cholerae serotype O1 | |
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | - |
Bacillus anthracis | |
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | - |
Campylobacter jejuni | |
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | - |
Clostridium perfringens | |
N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | - |
Vibrio cholerae serotype O1 | |
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | - |
Bacillus anthracis | |
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | - |
Campylobacter jejuni | |
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | - |
Clostridium perfringens | |
N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | - |
Vibrio cholerae serotype O1 | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Bacillus anthracis | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Campylobacter jejuni | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Clostridium perfringens | |
N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | - |
Vibrio cholerae serotype O1 | |
NAD+ | substrate inhibition | Bacillus anthracis | |
NAD+ | substrate inhibition | Campylobacter jejuni | |
NAD+ | substrate inhibition | Clostridium perfringens | |
NAD+ | substrate inhibition | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | |
0.049 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | |
0.055 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | |
0.061 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | |
0.064 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | |
0.08 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Vibrio cholerae serotype O1 | |
0.087 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL | Vibrio cholerae serotype O1 | |
0.1 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | |
0.15 | - |
IMP | pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | |
0.22 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | |
0.37 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | |
0.46 | - |
NAD+ | pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | |
0.51 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | |
0.52 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | |
0.55 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | |
0.56 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | |
1.1 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL | Vibrio cholerae serotype O1 | |
1.2 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51943 | - |
- |
Vibrio cholerae serotype O1 |
52093 | - |
- |
Clostridium perfringens |
52151 | - |
- |
Campylobacter jejuni |
52374 | - |
- |
Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | Clostridium perfringens | - |
XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Vibrio cholerae serotype O1 | - |
XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Campylobacter jejuni | - |
XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | Bacillus anthracis | - |
XMP + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Campylobacter jejuni | - |
- |
- |
Clostridium perfringens | - |
- |
- |
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged long and short enzyme CBS-deletion mutants, BaIMPDHDELTAL and BaIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis | Bacillus anthracis |
recombinant His-tagged long enzyme CBS-deletion mutant, ClpIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis | Clostridium perfringens |
recombinant His-tagged long enzyme CBS-deletion mutant, VcIMPDHDELTAL, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis | Vibrio cholerae serotype O1 |
recombinant His-tagged short enzyme CBS-deletion mutant, CjIMPDHDELTAS, from Escherichia coli by nickel affinity chromatography , tag cleavage by TEV protease, and removal by another step of nickel affinity chromatography, followed by dialysis | Campylobacter jejuni |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + NAD+ + H2O = XMP + NADH + H+ | the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP | Clostridium perfringens | |
IMP + NAD+ + H2O = XMP + NADH + H+ | the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP | Vibrio cholerae serotype O1 | |
IMP + NAD+ + H2O = XMP + NADH + H+ | the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP | Campylobacter jejuni | |
IMP + NAD+ + H2O = XMP + NADH + H+ | the IMPDH reaction involves two chemical transformations. First, the catalytic Cys attacks IMP, and hydride is transferred to NAD to form the covalent intermediate E-XMP*. In the second step, E-XMP* is hydrolyzed to produce XMP | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
IMP + NAD+ + H2O | - |
Clostridium perfringens | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Vibrio cholerae serotype O1 | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Campylobacter jejuni | XMP + NADH + H+ | - |
? | |
IMP + NAD+ + H2O | - |
Bacillus anthracis | XMP + NADH + H+ | - |
? | |
additional information | IMP- and XMP-enzyme binding structure analysis, overview | Vibrio cholerae serotype O1 | ? | - |
? | |
additional information | IMP-enzyme binding structure analysis, overview | Clostridium perfringens | ? | - |
? | |
additional information | IMP-enzyme binding structure analysis, overview | Campylobacter jejuni | ? | - |
? | |
additional information | IMP-enzyme binding structure analysis, overview | Bacillus anthracis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37929, long enzyme truncation CBS-mutant VcIMPDHDELTAL, sequence calculation | Vibrio cholerae serotype O1 |
tetramer | 4 * 38123, long enzyme truncation CBS-mutant ClpIMPDHDELTAL, sequence calculation | Clostridium perfringens |
tetramer | 4 * 40435, long enzyme truncation CBS-mutant BaIMPDHDELTAL, sequence calculation, 4 * 37920, short enzyme truncation CBS-mutant BaIMPDHDELTAL, sequence calculation | Bacillus anthracis |
tetramer | 4 * 40722, short enzyme truncation CBS-mutant CjIMPDHDELTAS, sequence calculation | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
IMP dehydrogenase | - |
Clostridium perfringens |
IMP dehydrogenase | - |
Vibrio cholerae serotype O1 |
IMP dehydrogenase | - |
Campylobacter jejuni |
IMP dehydrogenase | - |
Bacillus anthracis |
IMPDH | - |
Clostridium perfringens |
IMPDH | - |
Vibrio cholerae serotype O1 |
IMPDH | - |
Campylobacter jejuni |
IMPDH | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Clostridium perfringens |
25 | - |
assay at | Vibrio cholerae serotype O1 |
25 | - |
assay at | Campylobacter jejuni |
25 | - |
assay at | Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | |
1.9 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | |
2.1 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Vibrio cholerae serotype O1 | |
2.2 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | |
2.9 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | |
4.5 | - |
IMP | pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | |
5.2 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL | Vibrio cholerae serotype O1 | |
5.3 | - |
IMP | pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | |
6.1 | - |
IMP | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Clostridium perfringens |
8 | - |
assay at | Vibrio cholerae serotype O1 |
8 | - |
assay at | Campylobacter jejuni |
8 | - |
assay at | Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme binding structure analysis, overview | Clostridium perfringens | |
NAD+ | enzyme binding structure analysis, overview | Vibrio cholerae serotype O1 | |
NAD+ | enzyme binding structure analysis, overview | Campylobacter jejuni | |
NAD+ | enzyme binding structure analysis, overview | Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
NAD+ | pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | |
3.9 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | |
5.3 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | |
6.4 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | |
9 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | |
13 | - |
NAD+ | pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | |
13 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | |
13 | - |
NAD+ | pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant VcIMPDHDELTAL | Vibrio cholerae serotype O1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
the inhibitors all exhibit inhibitory potencies with IC50 values of over 0.005 mM with wild-type and truncation mutant | Vibrio cholerae serotype O1 | additional information | |
0.000006 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.00001 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000013 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000015 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.000019 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000019 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.00002 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000022 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000023 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000024 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000027 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000028 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000029 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000034 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000036 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.000038 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000039 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.00004 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000042 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000043 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.000044 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.000046 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000047 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000051 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000054 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.000063 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.000065 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.000066 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | N-(4-bromophenyl)-N-[1-[3-[1-(hydroxyimino)ethyl]phenyl]-1-methylethyl] urea | |
0.000067 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | N-(4-bromophenyl)-N-[(1-methylethenyl)phenyl]ethyl urea | |
0.000067 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000072 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000074 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.000092 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | 2-chloro-N-methyl-5-[[[1-methyl-1-[3-(1-methylethenyl)phenyl]ethyl]amino]carbonyl]aminobenzamide | |
0.000095 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | 2-(1-naphthalenyloxy)-N-[(2-(4-pyridinyl)-5-benzoxazolyl)]-(2S)-propanamide | |
0.0001 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.000107 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant CjIMPDHDELTAS | Campylobacter jejuni | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.000137 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant BaIMPDHDELTAS | Bacillus anthracis | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.00014 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.000169 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Campylobacter jejuni | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.0002 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant3 CjIMPDHDELTAS | Campylobacter jejuni | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.000364 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | 4-[(1R)-[1-(4-chlorophenyl)-1H-1,2,3-triazol-4yl]ethoxy]quinoline-1-oxide | |
0.00045 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.00057 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | N-(naphthalen-2-yl)-2-[2-(pyridin-2-yl)-1H-benzimidazol-1-yl]acetamide | |
0.00067 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Bacillus anthracis | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.0007 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant BaIMPDHDELTAL | Bacillus anthracis | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.0008 | - |
pH 8.0, 25°C, recombinant short enzyme CBS-deletion mutant3 BaIMPDHDELTAS | Bacillus anthracis | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.0017 | - |
pH 8.0, 25°C, recombinant wild-type enzyme | Clostridium perfringens | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide | |
0.0034 | - |
pH 8.0, 25°C, recombinant long enzyme CBS-deletion mutant ClpIMPDHDELTAL | Clostridium perfringens | 3,4-dihydro-3-methyl-4-oxo-N-(6,7,8,9-tetrahydro2-dibenzofuranyl)-1-phthalazineacetamide |
General Information | Comment | Organism |
---|---|---|
malfunction | the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation | Clostridium perfringens |
malfunction | the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation | Vibrio cholerae serotype O1 |
malfunction | the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation | Campylobacter jejuni |
malfunction | the inhibition of IMPDH leads to the depletion of the guanine nucleotide pool, which blocks proliferation | Bacillus anthracis |
metabolism | the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis | Clostridium perfringens |
metabolism | the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis | Vibrio cholerae serotype O1 |
metabolism | the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis | Campylobacter jejuni |
metabolism | the enzyme catalyzes the first and rate-limiting step in guanine nucleotide biosynthesis | Bacillus anthracis |
additional information | the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* | Clostridium perfringens |
additional information | the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* | Vibrio cholerae serotype O1 |
additional information | the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* | Campylobacter jejuni |
additional information | the enzyme has two essential but mutually exclusive conformations, an open conformation that accommodates both the substrate and cofactor during the dehydrogenase step, and a closed conformation where a mobile flap (referred to as the active site flap) moves into the cofactor-binding site for the hydrolysis of E-XMP* | Bacillus anthracis |