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Literature summary for 1.1.1.205 extracted from

  • Bessho, T.; Morii, S.; Kusumoto, T.; Shinohara, T.; Noda, M.; Uchiyama, S.; Shuto, S.; Nishimura, S.; Djikeng, A.; Duszenko, M.; Martin, S.K.; Inui, T.; Kubata, K.B.
    Characterization of the novel Trypanosoma brucei inosine 5-monophosphate dehydrogenase (2013), Parasitology, 140, 735-745.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
mizoribine 5'-monophosphate
-
Trypanosoma brucei
Mycophenolic acid
-
Trypanosoma brucei
NAD+ substrate inhibition Trypanosoma brucei
ribavirin 5'-monophosphate
-
Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
Inosine 5'-diphosphate pH 8.0, 25°C Trypanosoma brucei
1.3
-
NAD+ pH 8.0, 25°C Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
7 * 57000, SDS-PAGE Trypanosoma brucei
389000
-
sedimentation velocity analysis Trypanosoma brucei
420000
-
gel fitlration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-diphosphate + NAD+ + H2O Trypanosoma brucei
-
xanthosine 5'-diphosphate + NADH + H+ xanthosine 5'-diphosphate is the only product ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q387Q3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-diphosphate + NAD+ + H2O
-
Trypanosoma brucei xanthosine 5'-diphosphate + NADH + H+ xanthosine 5'-diphosphate is the only product ?

Subunits

Subunits Comment Organism
heptamer 7 * 57000, SDS-PAGE Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
Inosine 5'-diphosphate pH 8.0, 25°C Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0032
-
ribavirin 5'-monophosphate pH 8.0, 25°C Trypanosoma brucei
0.0033
-
mizoribine 5'-monophosphate pH 8.0, 25°C Trypanosoma brucei
0.021
-
Mycophenolic acid pH 8.0, 25°C Trypanosoma brucei
3
-
NAD+ pH 8.0, 25°C Trypanosoma brucei