Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K+ | the K+ dependence of kcat value derives from the rate of flap closure, which increases by more than 65fold in the presence of K+. When K+ is replaced with a dummy ion, the residues of the K+ binding site relax into ordered secondary structure, creating a barrier to conformational exchange. K+ mobilizes these residues by providing alternate interactions for the main chain carbonyls. So K+ changes the shape of the energy well, shrinking the reaction coordinate by shifting the closed conformation toward the open state | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NAD+ | presence of 1 mM K+, pH 8.0, 25°C | Cryptosporidium parvum | |
0.19 | - |
3-acetylpyridine adenine dinucleotide | presence of 100 mM K+, pH 8.0, 25°C | Cryptosporidium parvum | |
0.22 | - |
3-acetylpyridine adenine dinucleotide | absence of K+, pH 8.0, 25°C | Cryptosporidium parvum | |
1.4 | - |
NAD+ | absence of K+, pH 8.0, 25°C | Cryptosporidium parvum | |
2.4 | - |
NAD+ | presence of 1 mM K+, pH 8.0, 25°C | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | Q8T6T2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-diphosphate + NAD+ + H2O | - |
Cryptosporidium parvum | xanthosine 5'-diphosphate + NADH + H+ | - |
? | |
inosine 5'-phosphate + 3-acetylpyridine adenine dinucleotide + H2O | - |
Cryptosporidium parvum | xanthosine 5'-phosphate + reduced 3-acetylpyridine adenine dinucleotide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
3-acetylpyridine adenine dinucleotide | absence of K+, pH 8.0, 25°C | Cryptosporidium parvum | |
0.27 | - |
NAD+ | absence of K+, pH 8.0, 25°C | Cryptosporidium parvum | |
0.8 | - |
NAD+ | presence of 1 mM K+, pH 8.0, 25°C | Cryptosporidium parvum | |
2.6 | - |
NAD+ | presence of 1 mM K+, pH 8.0, 25°C | Cryptosporidium parvum | |
3 | - |
3-acetylpyridine adenine dinucleotide | presence of 100 mM K+, pH 8.0, 25°C | Cryptosporidium parvum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | - |
Cryptosporidium parvum | |
NAD+ | - |
Cryptosporidium parvum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
NAD+ | absence of K+, pH 8.0, 25°C | Cryptosporidium parvum | |
1.5 | - |
NAD+ | presence of 1 mM K+, pH 8.0, 25°C | Cryptosporidium parvum | |
4.9 | - |
NAD+ | presence of 100 mM K+, pH 8.0, 25°C | Cryptosporidium parvum |