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Literature summary for 1.1.1.205 extracted from

  • Guillen Schlippe, Y.V.; Hedstrom, L.
    Is Arg418 the catalytic base required for the hydrolysis step of the IMP dehydrogenase reaction? (2005), Biochemistry, 44, 11700-11707.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
R418H site-directed mutagenesis, altered kinetics compared to the wild-type enzyme Tritrichomonas suis
R418K site-directed mutagenesis, altered kinetics compared to the wild-type enzyme Tritrichomonas suis
R418Q site-directed mutagenesis, altered kinetics compared to the wild-type enzyme Tritrichomonas suis
T312A site-directed mutagenesis, altered kinetics compared to the wild-type enzyme Tritrichomonas suis
Y419F site-directed mutagenesis, altered kinetics compared to the wild-type enzyme Tritrichomonas suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information detailed kinetics, pre-steady-state kinetics, mechanistic rate constants, wild-type enzyme and mutant enzymes, overview Tritrichomonas suis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O Tritrichomonas suis
-
xanthosine 5'-phosphate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Tritrichomonas suis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
IMP + NAD+ + H2O = XMP + NADH + H+ covalent enzyme reaction intermediate, hydrolysis of enzyme-XMP is rate-limiting and requires the adoption of the closed conformation, Arg418 is involved in the reaction mechanism as catalytic base, Thr321 is involved in both hydride transfer and hydrolysis Tritrichomonas suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine 5'-phosphate + NAD+ + H2O
-
Tritrichomonas suis xanthosine 5'-phosphate + NADH
-
r

Synonyms

Synonyms Comment Organism
IMPDH
-
Tritrichomonas suis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Tritrichomonas suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Tritrichomonas suis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH-dependence of wild-type enzyme and mutant Y419F, overview Tritrichomonas suis
5.5 9
-
Tritrichomonas suis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Tritrichomonas suis
NADH
-
Tritrichomonas suis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information multi-inhibitor kinetics Tritrichomonas suis