Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | competitive with respect to IMP | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | Escherichia coli | - |
xanthosine 5'-phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
wild type and guaA mutants | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Escherichia coli |
native enzyme about 14fold by affinity chromatography on different immobilized dyes, elution by salt gradient, over 90% purity of the enzyme, method development and optimization, overview | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 58000, identical subunits | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
IMP:NAD+ oxidoreductase | - |
Escherichia coli |
inosine 5 -monophosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.6 | assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |