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Literature summary for 1.1.1.202 extracted from
- Utilization of glycerol as a hydrogen acceptor by Lactobacillus reuteri: purification of 1,3-propanediol:NAD+ oxidoreductase (1990), Appl. Environ. Microbiol., 56, 943-948.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | with 100 mM Na+, Li+, Mg2+ or NH4+ in addition to K+: 13-23% decrease in activity | Limosilactobacillus reuteri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
NAD+ | - |
Limosilactobacillus reuteri |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | highest levels of activity in presence of 100 mM K+ | Limosilactobacillus reuteri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, SDS-PAGE | Limosilactobacillus reuteri |
| 180000 | - |
gel filtration | Limosilactobacillus reuteri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propane-1,3-diol + NAD+ | Limosilactobacillus reuteri | - |
3-hydroxypropanal + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus reuteri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Limosilactobacillus reuteri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propane-1,3-diol + NAD+ | - |
Limosilactobacillus reuteri | 3-hydroxypropanal + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Limosilactobacillus reuteri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Limosilactobacillus reuteri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
3-hydroxypropanal + NADH | Limosilactobacillus reuteri |
| 7 | - |
propane-1,3-diol + NAD+ | Limosilactobacillus reuteri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Limosilactobacillus reuteri | |
| NADH | - |
Limosilactobacillus reuteri | |
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Release 2023.1 (January 2023)
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