Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | complete inhibition at 0.05 mM | Blautia producta | |
p-chloromercuribenzoic acid | complete inhibition at 0.001 mM | Blautia producta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
3alpha,7beta-dihydroxy-5beta-cholanoic acid | - |
Blautia producta | |
0.1 | - |
NADP+ | - |
Blautia producta | |
0.108 | - |
3alpha,7beta-dihydroxy-5beta-cholanoic acid | - |
Collinsella aerofaciens | |
0.238 | - |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine | - |
Blautia producta | |
0.286 | - |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine | - |
Blautia producta | |
0.4 | - |
NADP+ | - |
Collinsella aerofaciens | |
0.909 | - |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine | - |
Collinsella aerofaciens | |
1.639 | - |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine | - |
Collinsella aerofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Collinsella aerofaciens |
53000 | - |
gel filtration | Blautia producta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Collinsella aerofaciens | ursodeoxycholic acid | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Blautia producta | ursodeoxycholic acid | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Collinsella aerofaciens | only active on free and conjugated bile salts | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | Blautia producta | only active on free and conjugated bile salts | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ | Collinsella aerofaciens | - |
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ | Blautia producta | - |
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ | Collinsella aerofaciens | - |
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ | Blautia producta | - |
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blautia producta | - |
- |
- |
Collinsella aerofaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Collinsella aerofaciens |
to homogeneity, chromatography techniques | Blautia producta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Collinsella aerofaciens | - |
cell culture | - |
Blautia producta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
crude cell extract | Collinsella aerofaciens |
0.17 | - |
- |
Collinsella aerofaciens |
1.06 | - |
- |
Blautia producta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | ursodeoxycholic acid | Collinsella aerofaciens | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | ursodeoxycholic acid | Blautia producta | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | only active on free and conjugated bile salts | Collinsella aerofaciens | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ | only active on free and conjugated bile salts | Blautia producta | 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ | - |
Collinsella aerofaciens | 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ | - |
Blautia producta | 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ | - |
Collinsella aerofaciens | 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH | - |
? | |
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ | - |
Blautia producta | 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
50% loss of activity within 30 min | Blautia producta |
50 | - |
90% loss of activity within 3 min | Blautia producta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | 11 | - |
Collinsella aerofaciens |
9.5 | 10.5 | - |
Blautia producta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Collinsella aerofaciens | |
NADP+ | no activity with NAD+ | Blautia producta |