Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Prunus persica |
General Stability | Organism |
---|---|
enzyme thiolation with oxidized glutathione protects the enzyme from insolubilization caused by diamide, while incubation with NADP+ completely prevents enzyme precipitation | Prunus persica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | about 40% inhibition at 20 mM | Prunus persica | |
D-fructose 6-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
D-galactose 1-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
D-glucose 1-phosphate | about 55% inhibition at 20 mM | Prunus persica | |
D-ribose 5-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
Diamide | enzymatic activity decreases to approximately 7% at 500 mM diamide | Prunus persica | |
H2O2 | H2O2 oxidation causes enzyme inactivation in a time- and concentration-dependent manner | Prunus persica | |
mannose 1-phosphate | about 40% inhibition at 20 mM | Prunus persica | |
mannose 6-phosphate | about 30% inhibition at 20 mM | Prunus persica | |
additional information | no inhibition by D-trehalose 6-phosphate, D-fructose 2,6-bisphosphate, D-xylitol, D-glucose and D-fructose. Not affected by 5 mM GSSG | Prunus persica | |
phosphate | competitive inhibitor | Prunus persica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
NADPH | at pH 8.0 and 25°C | Prunus persica | |
0.059 | - |
NADP+ | at pH 8.0 and 25°C | Prunus persica | |
3.5 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Prunus persica | |
11 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Prunus persica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant effects are observed when the activity of the enzyme is assayed in the presence of 5 mM Mg2+ or Ca2+ | Prunus persica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Prunus persica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADPH + H+ | Prunus persica | - |
D-sorbitol 6-phosphate + NADP+ | - |
r | |
D-sorbitol 6-phosphate + NADP+ | Prunus persica | - |
D-glucose 6-phosphate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus persica | A5JUQ9 | cultivar Flordakin | - |
Oxidation Stability | Organism |
---|---|
NADP+ protects the enzyme from oxidative insolubilization | Prunus persica |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Prunus persica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Prunus persica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADPH + H+ | - |
Prunus persica | D-sorbitol 6-phosphate + NADP+ | - |
r | |
D-sorbitol 6-phosphate + NADP+ | - |
Prunus persica | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
additional information | almost no activity with mannose 6-phosphate or ribose 5-phosphate, no activity with NADH and NAD+ | Prunus persica | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Prunus persica |
homodimer | 2 * 34800, calculated from amino acid sequence | Prunus persica |
Synonyms | Comment | Organism |
---|---|---|
Ald6PRase | - |
Prunus persica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Prunus persica | |
NADPH | - |
Prunus persica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
9.07 | - |
D-glucose 1-phosphate | at pH 8.0 and 25°C | Prunus persica | |
26 | - |
D-fructose 1,6-bisphosphate | at pH 8.0 and 25°C | Prunus persica | |
29.6 | - |
phosphate | at pH 8.0 and 25°C | Prunus persica | |
55.4 | - |
D-fructose 6-phosphate | at pH 8.0 and 25°C | Prunus persica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Prunus persica | calculated from amino acid sequence | - |
7.9 |