KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
NADPH | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.071 | - |
NADP+ | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.091 | - |
phenylacetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.17 | - |
acetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.2 | - |
methylglyoxal | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
20 | - |
NAD+ | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
29.4 | - |
ethanol | 80°C, pH not specified in the publication | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1 gatom iron per subunit. The iron is present as a low-spin Fe(II) | Pyrococcus furiosus | |
Zinc | contains 1 gatom per subunit | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44840 | - |
6 * 44840, mass spectrometric analysis | Pyrococcus furiosus |
48000 | - |
6 * 48000, SDS-PAGE | Pyrococcus furiosus |
270000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADPH + H+ | Pyrococcus furiosus | the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways | ethanol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen sensitive, half-life in air is about 1 h at 23°C. It is stable under anaerobic conditions even at high temperature, with half-lives at 85 and 95°C of 160 and 7 h, respectively | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.3 | - |
80°C, pH not specified in the publication | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NADP+ | 169% of the activity compared to ethanol | Pyrococcus furiosus | butanal + NADPH + H+ | - |
r | |
1-heptanol + NADP+ | 86% of the activity compared to ethanol | Pyrococcus furiosus | heptanal + NADPH + H+ | - |
r | |
1-hexanol + NADP+ | 95% of the activity compared to ethanol | Pyrococcus furiosus | hexanal + NADPH + H+ | - |
r | |
1-octanol + NADP+ | 57% of the activity compared to ethanol | Pyrococcus furiosus | octanal + NADPH + H+ | - |
r | |
1-pentanol + NADP+ | 172% of the activity compared to ethanol | Pyrococcus furiosus | pentanal + NADPH + H+ | - |
r | |
1-propanol + NADP+ | 156% of the activity compared to ethanol | Pyrococcus furiosus | propanal + NADPH + H+ | - |
r | |
2-phenylethanol + NADP+ | 76% of the activity compared to ethanol | Pyrococcus furiosus | phenylacetaldehyde + NADPH + H+ | - |
r | |
acetaldehyde + NADPH + H+ | - |
Pyrococcus furiosus | ethanol + NADP+ | - |
r | |
acetaldehyde + NADPH + H+ | the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways | Pyrococcus furiosus | ethanol + NADP+ | - |
? | |
ethanol + NAD+ | kcat/Km for NAD+ is 1900fold lower compared to NADP+ | Pyrococcus furiosus | acetaldehyde + NADH + H+ | - |
r | |
ethanol + NADP+ | kcat/Km for NAD+ is 1900fold lower compared to NADP+ | Pyrococcus furiosus | acetaldehyde + NADPH + H+ | - |
r | |
isobutanol + NADP+ | 122% of the activity compared to ethanol | Pyrococcus furiosus | isobutanal + NADPH + H+ | - |
r | |
methylglyoxal + NADPH | 27% of the activity compared to ethanol | Pyrococcus furiosus | ? | - |
? | |
additional information | no activity with methanol, 2-propanol, glycerol, D-sorbitol | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 48000, SDS-PAGE | Pyrococcus furiosus |
homohexamer | 6 * 44840, mass spectrometric analysis | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
anaerobic conditions, 24 h, stable | Pyrococcus furiosus |
85 | - |
anaerobic conditions, half-life: 160 h | Pyrococcus furiosus |
95 | - |
anaerobic conditions, half-life: 7 h | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
NAD+ | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
3.7 | - |
methylglyoxal | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
5.5 | - |
acetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
6.3 | - |
NADPH | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
10.4 | - |
phenylacetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
15.8 | - |
NADP+ | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
19.2 | - |
ethanol | 80°C, pH not specified in the publication | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | 10.2 | 80°C, optimal pH for ethanol oxidation | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | kcat/Km for NAD+ is 1900fold lower compared to NADP+ | Pyrococcus furiosus | |
NADPH | - |
Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
acetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.12 | - |
NAD+ | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
0.65 | - |
ethanol | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
18.5 | - |
methylglyoxal | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
93 | - |
NADPH | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
114 | - |
phenylacetaldehyde | 80°C, pH not specified in the publication | Pyrococcus furiosus | |
223 | - |
NADP+ | 80°C, pH not specified in the publication | Pyrococcus furiosus |