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Literature summary for 1.1.1.2 extracted from

  • van der Oost, J.; Voorhorst, W.G.; Kengen, S.W.; Geerling, A.C.; Wittenhorst, V.; Gueguen, Y.; de Vos, W.M.
    Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (2001), Eur. J. Biochem., 268, 3062-3068.
    View publication on PubMed
Search for more literature for 1.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 NADPH pH 7.0, 70°C Pyrococcus furiosus
0.08
-
 NADP+ pH 10.0, 70°C Pyrococcus furiosus
3.3
-
 Pyruvaldehyde pH 7.0, 70°C Pyrococcus furiosus
60.8
-
 2-Pentanol pH 10.0, 70°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25962
-
 2 * 25962, calculated from sequence Pyrococcus furiosus
26000
-
 2 * 26000, SDS-PAGE Pyrococcus furiosus
50000 55000 gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus O73949
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Storage Stability

Storage Stability Organism
4°C, several months, stable Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-butanol + NADP+ the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol Pyrococcus furiosus 2-butanone + NADPH + H+
-
 ?
(S)-2-butanol + NADP+ the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol Pyrococcus furiosus 2-butanone + NADPH + H+
-
 ?
2-pentanol + NADP+ maximal activity in oxidative reaction Pyrococcus furiosus 2-pentanone + NADPH + H+
-
 r
2-propanol + NADP+
-
 Pyrococcus furiosus acetone + NADPH + H+
-
 ?
additional information a broad range of primary alcohols could be oxidized by AdhA and includes substrates with 2-10 carbon atoms. It shows activity towards a broad range of secondary alcohols that on average are oxidized faster than the primary alcohols. Alcohols with a second or a third hydroxyl group are converted less efficiently Pyrococcus furiosus ?
-
 ?
pyruvaldehyde + NADPH + H+ maximal activity in reductive reaction Pyrococcus furiosus lactaldehyde + NADP+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Pyrococcus furiosus
dimer 2 * 25962, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
AdhA
-
 Pyrococcus furiosus
short-chain ADH
-
 Pyrococcus furiosus
short-chain alcohol dehydrogenase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus
90
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: 150 h Pyrococcus furiosus
90
-
 half-life: 22.5 h Pyrococcus furiosus
100
-
 half-life: 25 min Pyrococcus furiosus
103
-
 melting temperature is 102.7°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 aldehyde reduction Pyrococcus furiosus
10 11 alcohol oxidation Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6.7 9 pH 6.7: about 80% of maximal activity, pH 9.0: about 80% of maximal activity Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pyrococcus furiosus
NADPH
-
 Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
 2-Pentanol pH 10.0, 70°C Pyrococcus furiosus
4.2
-
 Pyruvaldehyde pH 7.0, 70°C Pyrococcus furiosus