Cloned (Comment) | Organism |
---|---|
ADH gene cloned and overexpressed in Escherichia coli | Thermococcus zilligii |
gene encoding NADPH group III alcohol dehydrogenase cloned and overexpressed in Escherichia coli | Thermococcus hydrothermalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
benzaldehyde | co-substrate NADP | Thermococcus hydrothermalis | |
0.13 | - |
NADPH | benzaldehyde as substrate | Thermococcus hydrothermalis | |
0.8 | - |
NADP+ | benzyl aclohol as substrate | Thermococcus hydrothermalis | |
2 | - |
benzyl alcohol | co-substrate NADP | Thermococcus hydrothermalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
80500 | - |
pH-dependent enzyme structure, pH 7.5 | Thermococcus hydrothermalis |
197000 | - |
pH-dependent enzyme structure, pH 10.5 | Thermococcus hydrothermalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Archaeoglobus fulgidus | - |
- |
- |
Bacillus methanolicus | - |
- |
- |
Clostridium acetobutylicum | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Saccharomyces cerevisiae | - |
yeast | - |
Schizosaccharomyces pombe | - |
- |
- |
Thermococcus hydrothermalis | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Thermococcus zilligii | - |
- |
- |
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus hydrothermalis |
- |
Thermococcus zilligii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.66 | - |
native enzyme | Thermococcus hydrothermalis |
24 | - |
recombinant enzyme | Thermococcus hydrothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + NADP+ | - |
Archaeoglobus fulgidus | ? + NADPH | - |
? | |
1,3-propanediol + NADP+ | - |
Thermococcus litoralis | ? + NADPH | - |
? | |
benzaldehyde + NADPH + H+ | - |
Thermococcus hydrothermalis | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NADP+ | - |
Thermococcus hydrothermalis | benzaldehyde + NADPH + H+ | - |
r | |
glycerol + NADP+ | - |
Thermococcus hydrothermalis | DL-glyceraldehyde + NADPH + H+ | - |
r | |
methanol + NADP+ | - |
Thermococcus hydrothermalis | formaldehyde + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharolobus solfataricus |
dimer | - |
Thermococcus hydrothermalis |
tetramer | - |
Thermococcus litoralis |
tetramer | 4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
Synonyms | Comment | Organism |
---|---|---|
putative iron alcohol dehydrogenase | - |
Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimum for aldehyde reduction | Thermococcus hydrothermalis |
10.5 | - |
optimum for alcohol oxidation | Thermococcus hydrothermalis |