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Literature summary for 1.1.1.2 extracted from
- Cloning and over-expression in Escherichia coli of the gene encoding NADPH group III alcohol dehydrogenase from Thermococcus hydrothermalis. Characterization and comparison of the native and the recombinant enzymes (1999), Eur. J. Biochem., 264, 880-889.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ADH gene cloned and overexpressed in Escherichia coli | Thermococcus zilligii |
| gene encoding NADPH group III alcohol dehydrogenase cloned and overexpressed in Escherichia coli | Thermococcus hydrothermalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
benzaldehyde | co-substrate NADP | Thermococcus hydrothermalis |  |
| 0.13 | - |
NADPH | benzaldehyde as substrate | Thermococcus hydrothermalis | |
| 0.8 | - |
NADP+ | benzyl aclohol as substrate | Thermococcus hydrothermalis | |
| 2 | - |
benzyl alcohol | co-substrate NADP | Thermococcus hydrothermalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
| 80500 | - |
pH-dependent enzyme structure, pH 7.5 | Thermococcus hydrothermalis |
| 197000 | - |
pH-dependent enzyme structure, pH 10.5 | Thermococcus hydrothermalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
| Archaeoglobus fulgidus | - |
- |
- |
| Bacillus methanolicus | - |
- |
- |
| Clostridium acetobutylicum | - |
- |
- |
| Pyrococcus furiosus | - |
- |
- |
| Saccharomyces cerevisiae | - |
yeast | - |
| Schizosaccharomyces pombe | - |
- |
- |
| Thermococcus hydrothermalis | - |
- |
- |
| Thermococcus litoralis | - |
- |
- |
| Thermococcus zilligii | - |
- |
- |
| Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus hydrothermalis |
- |
Thermococcus zilligii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.66 | - |
native enzyme | Thermococcus hydrothermalis |
| 24 | - |
recombinant enzyme | Thermococcus hydrothermalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-propanediol + NADP+ | - |
Archaeoglobus fulgidus | ? + NADPH | - |
? | |
| 1,3-propanediol + NADP+ | - |
Thermococcus litoralis | ? + NADPH | - |
? | |
| benzaldehyde + NADPH + H+ | - |
Thermococcus hydrothermalis | benzyl alcohol + NADP+ | - |
r | |
| benzyl alcohol + NADP+ | - |
Thermococcus hydrothermalis | benzaldehyde + NADPH + H+ | - |
r | |
| glycerol + NADP+ | - |
Thermococcus hydrothermalis | DL-glyceraldehyde + NADPH + H+ | - |
r | |
| methanol + NADP+ | - |
Thermococcus hydrothermalis | formaldehyde + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharolobus solfataricus |
| dimer | - |
Thermococcus hydrothermalis |
| tetramer | - |
Thermococcus litoralis |
| tetramer | 4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| putative iron alcohol dehydrogenase | - |
Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
optimum for aldehyde reduction | Thermococcus hydrothermalis |
| 10.5 | - |
optimum for alcohol oxidation | Thermococcus hydrothermalis |
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