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Literature summary for 1.1.1.198 extracted from

  • Khine, A.A.; Lu, P.C.; Ko, T.P.; Huang, K.F.; Chen, H.P.
    Cloning, expression, identification and characterization of borneol dehydrogenase isozymes in Pseudomonas sp. TCU-HL1 (2020), Protein Expr. Purif., 175, 105715 .
    View publication on PubMed

Application

Application Comment Organism
synthesis production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium Pseudomonas sp. TCU-HL1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp. TCU-HL1

Inhibitors

Inhibitors Comment Organism Structure
(+)-Camphor
-
Pseudomonas sp. TCU-HL1
(-)-Camphor
-
Pseudomonas sp. TCU-HL1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
(+)-borneol isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.09
-
(+)-borneol soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.09
-
(+)-borneol soluble recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
0.12
-
(-)-borneol refolded isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.12
-
(+)-borneol refolded isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.12
-
(+)-borneol refolded recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
0.21
-
(-)-borneol soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
1.5
-
(-)-borneol isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Pseudomonas sp. TCU-HL1
-
(+)-camphor + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. TCU-HL1
-
-
-
Pseudomonas sp. TCU-HL1 A0A1B3EB36 isoform BDH1
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography Pseudomonas sp. TCU-HL1
production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium Pseudomonas sp. TCU-HL1

Renatured (Commentary)

Renatured (Comment) Organism
-
Pseudomonas sp. TCU-HL1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
Pseudomonas sp. TCU-HL1 (+)-camphor + NADH + H+
-
?
(+)-borneol + NAD+ the enzyme prefers to degrade (+)-borneol, rather than (-)-borneol Pseudomonas sp. TCU-HL1 (+)-camphor + NADH + H+
-
?
(-)-borneol + NAD+
-
Pseudomonas sp. TCU-HL1 (-)-camphor + NADH + H+
-
?
additional information enzyme additionally catalyzes the reaction with (-)-borneol, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate Pseudomonas sp. TCU-HL1 ?
-
-

Subunits

Subunits Comment Organism
? x * about 30000, isoform BDH1, SDS-PAGE Pseudomonas sp. TCU-HL1
? x * about 35000, isoform BDH2, SDS-PAGE Pseudomonas sp. TCU-HL1

Synonyms

Synonyms Comment Organism
BDH1
-
Pseudomonas sp. TCU-HL1
BDH1 isoform Pseudomonas sp. TCU-HL1
BDH2 isoform Pseudomonas sp. TCU-HL1
THL1_4180
-
Pseudomonas sp. TCU-HL1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
(-)-borneol refolded isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.5
-
(+)-borneol refolded isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.5
-
(+)-borneol refolded recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
12.4
-
(-)-borneol soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
17
-
(+)-borneol soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
17
-
(+)-borneol soluble recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
18.5
-
(-)-borneol isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
27.8
-
(+)-borneol isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp. TCU-HL1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07
-
(+)-Camphor soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.07
-
(+)-Camphor soluble recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
0.08
-
(-)-Camphor soluble isoform BDH1, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.16
-
(+)-Camphor isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1
0.16
-
(+)-Camphor refolded recombinant protein, pH 7, 25┬░C Pseudomonas sp. TCU-HL1
0.52
-
(-)-Camphor isoform BDH2, at pH 7.0, temperature not specified in the publication Pseudomonas sp. TCU-HL1