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Literature summary for 1.1.1.198 extracted from

  • Ma, R.; Su, P.; Jin, B.; Guo, J.; Tian, M.; Mao, L.; Tang, J.; Chen, T.; Lai, C.; Zeng, W.; Cui, G.; Huang, L.
    Molecular cloning and functional identification of a high-efficiency (+)-borneol dehydrogenase from Cinnamomum camphora (L.) Presl (2021), Plant Physiol. Biochem., 158, 363-371 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Transetta (DE3) cells Cinnamomum camphora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0251
-
(+)-borneol isoform BDH3, at pH 8.5 and 30┬░C Cinnamomum camphora
0.0369
-
(-)-borneol isoform BDH3, at pH 9.0 and 32┬░C Cinnamomum camphora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Cinnamomum camphora
-
(+)-camphor + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Cinnamomum camphora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Cinnamomum camphora

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Cinnamomum camphora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
Cinnamomum camphora (+)-camphor + NADH + H+
-
?
(-)-borneol + NAD+
-
Cinnamomum camphora (-)-camphor + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
BDH3
-
Cinnamomum camphora

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
9
-
highest activity value for (-)-borneol at 32┬░C Cinnamomum camphora
30
-
highest activity value for (+)-borneol at pH 30┬░C Cinnamomum camphora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0021
-
(-)-borneol isoform BDH3, at pH 9.0 and 32┬░C Cinnamomum camphora
0.0054
-
(+)-borneol isoform BDH3, at pH 8.5 and 30┬░C Cinnamomum camphora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
highest activity value for (+)-borneol at pH 8.5 Cinnamomum camphora
9
-
highest activity value for (-)-borneol at pH 9.0 Cinnamomum camphora

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cinnamomum camphora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.056
-
(-)-borneol isoform BDH3, at pH 9.0 and 32┬░C Cinnamomum camphora
0.22
-
(+)-borneol isoform BDH3, at pH 8.5 and 30┬░C Cinnamomum camphora