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Literature summary for 1.1.1.198 extracted from

  • Drienovska, I.; Kolanovic, D.; Chanique, A.; Sieber, V.; Hofer, M.; Kourist, R.
    Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L (2020), Phytochemistry, 172, 112227 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salvia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
NAD+ isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis
0.16
-
(+)-borneol isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
124800
-
gel filtration Salvia officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Salvia officinalis
-
(+)-camphor + NADH + H+
-
?
(+/-)-isoborneol + NAD+ Salvia officinalis
-
(-)-camphor + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Salvia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + (-)-isoborneol + NAD+ the reaction is catalyzed by isoform BDH1 Salvia officinalis (+)-camphor + (-)-isoborneol + NADH + H+
-
?
(+)-borneol + NAD+
-
Salvia officinalis (+)-camphor + NADH + H+
-
?
(+)-borneol + NAD+ the reaction is catalyzed by isoform BDH2 Salvia officinalis (+)-camphor + NADH + H+
-
?
(+/-)-isoborneol + NAD+
-
Salvia officinalis (-)-camphor + NADH + H+
-
?
(+/-)-isoborneol + NAD+ the reaction is catalyzed by isoform BDH2 Salvia officinalis (-)-camphor + NADH + H+
-
?
(-)-borneol + NAD+ the reaction is catalyzed by isoform BDH2 Salvia officinalis (-)-camphor + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
BDH1 isoform Salvia officinalis
BDH2 isoform Salvia officinalis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60
-
-
Salvia officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0049
-
NAD+ isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis
0.005
-
(+)-borneol isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Salvia officinalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Salvia officinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.031
-
(+)-borneol isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis
0.102
-
NAD+ isoform BDH2, at pH 8.0 and 20┬░C Salvia officinalis