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Literature summary for 1.1.1.198 extracted from

  • Chanique, A.; Dimos, N.; Drienovska, I.; Calderini, E.; Pantin, M.; Helmer, C.; Hofer, M.; Sieber, V.; Parra, L.; Loll, B.; Kourist, R.
    A structural view on the stereospecificity of plant borneol-type dehydrogenases (2021), ChemCatChem, 13, 2262-2277 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salvia rosmarinus
expression in Escherichia coli Salvia officinalis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M HEPES/NaOH pH 7.0 to pH 7.8, 5/4 pentaerythritol propoxylate 25% (v/v), 30%, 35%, 0.1 M and 0.2 M KCl Salvia rosmarinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Salvia rosmarinus
-
(+)-camphor + NADH + H+
-
?
(+)-borneol + NAD+ Pseudomonas sp. TCU-HL1
-
(+)-camphor + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. TCU-HL1
-
-
-
Salvia officinalis
-
-
-
Salvia rosmarinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Salvia rosmarinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0029
-
with (-)-carveol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0032
-
with propan-1-ol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.0033
-
with cyclohexanol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0033
-
with propan-1-ol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0039
-
with (+)-endo-norborneol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0044
-
with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0046
-
with (+)-fenchol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0048
-
with (-)-exo-norborneol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.005
-
with (-)-carveol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.0062
-
with heptan-2-ol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.0083
-
with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.0098
-
with heptan-2-ol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.01
-
with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.011
-
with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.013
-
with octan-3-ol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.018
-
with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.023
-
with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.024
-
with (+)-isoborneol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.028
-
with (+)-endo-norborneol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.028
-
with cyclohexanol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.03
-
with (+)-fenchol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.03
-
with (+/-)-borneol as substrate, at pH 7.5 and 25┬░C Salvia rosmarinus
0.043
-
with octan-3-ol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.069
-
with (-)-exo-norborneol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.115
-
with (+)-isoborneol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
0.122
-
with (+/-)-borneol as substrate, at pH 7.5 and 25┬░C Pseudomonas sp. TCU-HL1
9.8
-
isoform BDH1, substrate 2-hydroxyheptanol, pH 9, temperature not specified in the publication Salvia officinalis
10
-
isoform BDH1, substrate 2-methylcyclohexanol, pH 9, temperature not specified in the publication Salvia officinalis
10
-
isoform BDH2, substrate rac-borneol, pH 9, temperature not specified in the publication Salvia officinalis
13
-
isoform BDH1, substrate 6-hydroxyoctanol, pH 9, temperature not specified in the publication Salvia officinalis
16
-
isoform BDH2, substrate (-)-carveol, pH 9, temperature not specified in the publication Salvia officinalis
24
-
isoform BDH1, substrate isoborneol, pH 9, temperature not specified in the publication Salvia officinalis
30
-
isoform BDH1, substrate rac-borneol, pH 9, temperature not specified in the publication Salvia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
Salvia officinalis (+)-camphor + NADH + H+
-
?
(+)-borneol + NAD+
-
Salvia rosmarinus (+)-camphor + NADH + H+
-
?
(+)-borneol + NAD+
-
Pseudomonas sp. TCU-HL1 (+)-camphor + NADH + H+
-
?
(+)-endo-norborneol + NAD+
-
Salvia rosmarinus (-)-exonorcamphor + NADH + H+
-
?
(+)-endo-norborneol + NAD+
-
Pseudomonas sp. TCU-HL1 (-)-exonorcamphor + NADH + H+
-
?
(+)-fenchol + NAD+
-
Salvia rosmarinus (-)-fenchone + NADH + H+
-
?
(+)-fenchol + NAD+
-
Pseudomonas sp. TCU-HL1 (-)-fenchone + NADH + H+
-
?
(+)-isoborneol + NAD+
-
Salvia rosmarinus (-)-camphor + NADH + H+
-
?
(+)-isoborneol + NAD+
-
Pseudomonas sp. TCU-HL1 (-)-camphor + NADH + H+
-
?
(+/-)-borneol + NAD+
-
Salvia rosmarinus (+/-)-camphor + NADH + H+
-
?
(+/-)-borneol + NAD+
-
Pseudomonas sp. TCU-HL1 (+/-)-camphor + NADH + H+
-
?
(-)-carveol + NAD+
-
Salvia rosmarinus ?
-
?
(-)-carveol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
(-)-cis-carveol + NAD+
-
Salvia officinalis (-)-carvone + NADH + H+
-
?
(-)-exo-norborneol + NAD+
-
Salvia rosmarinus (+)-exonorcamphor + NADH + H+
-
?
(-)-exo-norborneol + NAD+
-
Pseudomonas sp. TCU-HL1 (+)-exonorcamphor + NADH + H+
-
?
2-hydroxyheptanol + NAD+
-
Salvia officinalis 2-oxoheptanol + NADH + H+
-
?
2-methyl cyclohexanol + NAD+
-
Salvia rosmarinus ?
-
?
2-methyl cyclohexanol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
2-methylcyclohexanol + NAD+
-
Salvia officinalis 2-methylcyclohexanone + NADH + H+
-
?
3-hydroxyoctanol + NAD+
-
Salvia officinalis 3-oxooctanol + NADH + H+
-
?
3-methyl cyclohexanol + NAD+
-
Salvia officinalis 3-methycyclohexanone + NADH + H+
-
?
3-methyl cyclohexanol + NAD+
-
Salvia rosmarinus ?
-
?
3-methyl cyclohexanol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
4-methyl cyclohexanol + NAD+
-
Salvia rosmarinus ?
-
?
4-methyl cyclohexanol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
cyclohexanol + NAD+
-
Salvia rosmarinus ?
-
?
cyclohexanol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
heptan-2-ol + NAD+
-
Salvia rosmarinus ?
-
?
heptan-2-ol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
additional information isoforms BDH1 and BDH2 also accept monocyclic and linear substrates, clearly favoring secondary alcohols over primary alcohols. No substrate: menthol Salvia officinalis ?
-
-
additional information no activity with menthol Salvia rosmarinus ?
-
-
additional information no activity with menthol Pseudomonas sp. TCU-HL1 ?
-
-
octan-3-ol + NAD+
-
Salvia rosmarinus ?
-
?
octan-3-ol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?
propan-1-ol + NAD+
-
Salvia rosmarinus ?
-
?
propan-1-ol + NAD+
-
Pseudomonas sp. TCU-HL1 ?
-
?

Synonyms

Synonyms Comment Organism
BDH
-
Pseudomonas sp. TCU-HL1
BDH1
-
Salvia officinalis
BDH1
-
Salvia rosmarinus
BDH2
-
Salvia officinalis
borneol-type dehydrogenase 1
-
Salvia rosmarinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Salvia rosmarinus
7.5
-
-
Pseudomonas sp. TCU-HL1

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 isoform BDH1, 30 min, more than 50% residual activity Salvia officinalis
5 10.5 the enzyme retains over 50% activity for pH values between 5.0 and 10.5 after 30 min of incubation. After 24 h of incubation, the activity showed little variation for pH between 5.5 and 10.5 Salvia rosmarinus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Salvia rosmarinus
NAD+
-
Pseudomonas sp. TCU-HL1