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Literature summary for 1.1.1.198 extracted from

  • Hofer, M.; Diener, J.; Begander, B.; Kourist, R.; Sieber, V.
    Engineering of a borneol dehydrogenase from P. putida for the enzymatic resolution of camphor (2021), Appl. Microbiol. Biotechnol., 105, 3159-3167 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. ATCC 17453

Protein Variants

Protein Variants Comment Organism
E145G the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
E145L/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
E145M/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
E145M/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
E145S/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
E145S/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S145H the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S145L the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S145M the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S145S the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S146A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S146A/Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
S146A/Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
Y188A mutant catalyzes stereoselective reduction of racemic camphor with 23% conversion and 99% enantiomeric excess Pseudomonas putida
Y188A the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
Y188I the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
Y188L the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
Y188T mutant catalyzes stereoselective reduction of racemic camphor with 22% conversion and 99% enantiomeric excess Pseudomonas putida
Y188T the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453
Y188V the mutant shows a significant increase in (+)-camphor selectivity compared to the wild type enzyme Pseudomonas sp. ATCC 17453

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NAD+ wild type enzyme, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.06
-
NAD+ wild-type, pH 7.5, 30┬░C Pseudomonas putida
0.095
-
(+)-borneol wild-type, pH 7.5, 30┬░C Pseudomonas putida
0.095
-
(+)-borneol wild type enzyme, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.115
-
(-)-borneol wild type enzyme, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.28
-
(+)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.28
-
(+)-borneol mutant Y188A, pH 7.5, 30┬░C Pseudomonas putida
0.34
-
(-)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.46
-
(+)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.46
-
(+)-borneol mutant Y188T, pH 7.5, 30┬░C Pseudomonas putida
0.73
-
(-)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Pseudomonas sp. ATCC 17453
-
(+)-camphor + NADH + H+
-
r
(+)-camphor + NADH + H+ Pseudomonas sp. ATCC 17453
-
(+)-borneol + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ATCC 17453
-
-
-
Pseudomonas sp. ATCC 17453
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Pseudomonas sp. ATCC 17453

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
Pseudomonas putida (+)-camphor + NADH + H+
-
r
(+)-borneol + NAD+
-
Pseudomonas sp. ATCC 17453 (+)-camphor + NADH + H+
-
r
(+)-borneol + NAD+
-
Pseudomonas putida ATCC 17453 (+)-camphor + NADH + H+
-
r
(+)-camphor + NADH + H+
-
Pseudomonas putida (+)-borneol + NAD+
-
r
(+)-camphor + NADH + H+
-
Pseudomonas sp. ATCC 17453 (+)-borneol + NAD+
-
r
(+)-camphor + NADH + H+
-
Pseudomonas putida ATCC 17453 (+)-borneol + NAD+
-
r
(+)-isoborneol + NAD+
-
Pseudomonas putida (+)-isocamphor + NADH + H+
-
?
(+)-isoborneol + NAD+
-
Pseudomonas putida ATCC 17453 (+)-isocamphor + NADH + H+
-
?
(+/-)-isoborneol + NADH + H+
-
Pseudomonas sp. ATCC 17453 ?
-
?
(-)-borneol + NAD+
-
Pseudomonas sp. ATCC 17453 (-)-camphor + NADH + H+
-
?
(1R)-(+)-camphor + (1S)-(-)-camphor + NADH + H+
-
Pseudomonas sp. ATCC 17453 (+)-borneol + (1S)-(-)-camphor + NAD+
-
?
additional information enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ?
-
-
additional information isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, (+/-)-2-butanol, L-carveol, and DL-menthol are not accepted as substrates Pseudomonas sp. ATCC 17453 ?
-
-
additional information enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ATCC 17453 ?
-
-

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Pseudomonas sp. ATCC 17453
? x * 27500, calculated from amino acid sequence Pseudomonas sp. ATCC 17453
? x * 27500, calculated from sequence, x * 31000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
BDH
-
Pseudomonas sp. ATCC 17453
BDH1 cf. EC 1.1.1.227 Pseudomonas putida

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Pseudomonas putida
30
-
-
Pseudomonas sp. ATCC 17453

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
NAD+ at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.07
-
(-)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.13
-
(-)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.89
-
(+)-borneol wild-type, pH 7.5, 30┬░C Pseudomonas putida
0.89
-
(+)-borneol at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
0.97
-
(-)-borneol at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
1.51
-
(+)-borneol mutant enzyme S146A/Y188A, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
1.51
-
(+)-borneol mutant Y188A, pH 7.5, 30┬░C Pseudomonas putida
3.32
-
(+)-borneol mutant enzyme S146A/Y188T, at pH 7.5 and 30┬░C Pseudomonas sp. ATCC 17453
3.32
-
(+)-borneol mutant Y188T, pH 7.5, 30┬░C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
pH optimum for the reduction of camphor Pseudomonas sp. ATCC 17453
4.5
-
reduction of camphor Pseudomonas putida
7.5
-
oxidation of borneol Pseudomonas putida
7.5
-
pH optimum for the oxidation of (+/-)-borneol Pseudomonas sp. ATCC 17453

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp. ATCC 17453

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida calculated from sequence
-
5.4