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Literature summary for 1.1.1.198 extracted from

  • Croteau, R.; Hooper, C.L.; Felton, M.
    Biosynthesis of monoterpenes. Partial purification and characterization of a bicyclic monoterpenol dehydrogenase from sage (Salvia officinalis) (1978), Arch. Biochem. Biophys., 188, 182-193.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(+)-Camphor 27% inhibition at 0.5 mM Salvia officinalis
(-)-Thujol competitive inhibition of (+)-borneol oxidation Salvia officinalis
8-hydroxyquinoline 57% inhibition at 1 mM Salvia officinalis
Borate inhibition of borneol oxidation Salvia officinalis
Cu2+ 85% inhibition at 2 mM Salvia officinalis
ethylenediaminetetraacetate slight inhibition at 1 mM Salvia officinalis
Hg2+ nearly complete inhibition at 2 mM Salvia officinalis
iodoacetamide 41% inhibition at 1 mM Salvia officinalis
N-ethylmaleimide 53% inhibition at 1 mM Salvia officinalis
NaCN slight inhibition at 1 mM Salvia officinalis
NADH 46% inhibition at 1 mM Salvia officinalis
NaN3 slight inhibition at 1 mM Salvia officinalis
o-phenanthroline slight inhibition at 1 mM Salvia officinalis
p-hydroxymercuribenzoate 50% inhibition at 0.05 mM Salvia officinalis
Pb2+ 26% inhibition at 2 mM Salvia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
(+)-borneol
-
Salvia officinalis
0.041
-
(-)-Thujol
-
Salvia officinalis
0.07
-
NAD+
-
Salvia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
gel filtration Salvia officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Salvia officinalis B-site stereospecific, partially reversible (+)-camphor + NADH
-
r

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
sage
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, chromatography techniques, affinity chromatography Salvia officinalis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Salvia officinalis
-

Storage Stability

Storage Stability Organism
0Ā°C, 0.1 M sodium phosphate, pH 8.0, 15% glycerol, 20 mM 2-mercaptoethanol, 3 days, 25% loss of activity Salvia officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+ B-site stereospecific, partially reversible Salvia officinalis (+)-camphor + NADH
-
r
(+)-dihydrocarveol + NAD+ poor substrate Salvia officinalis (-)-dihydrocarvone + NADH
-
?
(+)-endo-fenchol + NAD+ 26% of the activity compared to (+)-borneol Salvia officinalis 1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
?
(+)-isoborneol + NAD+ nearly the same activity compared to (+)-borneol Salvia officinalis (+)-isocamphor + NADH
-
?
(-)-borneol + NAD+ 36% of the activity compared to (+)-borneol Salvia officinalis (-)-camphor + NADH + H+
-
?
(-)-endo-fenchol + NAD+ poor substrate Salvia officinalis 1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
?
(-)-isoborneol + NAD+ 30% of the activity compared to (+)-borneol Salvia officinalis (-)-isocamphor + NADH
-
?
(-)-neothujol + NAD+ 65% of the activity compared to (+)-borneol Salvia officinalis (-)-neothujone + NADH
-
?
(-)-thujol + NAD+ 2fold higher activity than for (+)-borneol Salvia officinalis (-)-thujone + NADH
-
?
(1R)-menthol + NAD+ poor substrate Salvia officinalis (1R)-trans-p-menthan-3-one + NADH
-
?
geraniol + NAD+ poor substrate Salvia officinalis geranial + NADH + H+
-
?
nerol + NAD+ poor substrate Salvia officinalis neryl aldehyde + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Salvia officinalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
most stable around Salvia officinalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Salvia officinalis
NADP+ 25% as efficient as NAD+ Salvia officinalis