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Literature summary for 1.1.1.195 extracted from
- The cinnamyl alcohol dehydrogenase gene family in melon (Cucumis melo L.): bioinformatic analysis and expression patterns (2014), PLoS ONE, 9, e101730.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CAD1, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00038, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real-time PCR expression analysis | Cucumis melo |
| gene CAD2, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00034, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis | Cucumis melo |
| gene CAD3, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold01596, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis | Cucumis melo |
| gene CAD4, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00005, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis | Cucumis melo |
| gene CAD5, DNA and amino acid sequence determination and analysis, genetic localization CM3.5_scaffold00059, intron-exon structure of melon CAD genes, promoter sequence analysis, phylogenetic analysis, semi-quantitative PCR and quantitative real time PCR expression analysis | Cucumis melo |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | no typical signal peptides are found in all CmCADs, CmCAD proteins may locate in the cytoplasm | Cucumis melo | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38420 | - |
CmCAD4 | Cucumis melo |
| 38870 | - |
CmCAD5 | Cucumis melo |
| 39200 | - |
CmCAD1 | Cucumis melo |
| 39400 | - |
CmCAD2 | Cucumis melo |
| 39620 | - |
CmCAD3 | Cucumis melo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cinnamyl alcohol + NADP+ | Cucumis melo | - |
cinnamaldehyde + NADPH + H+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis melo | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Cucumis melo | - |
| fruit | - |
Cucumis melo | - |
| additional information | differential expression of CmCADs | Cucumis melo | - |
| additional information | differential expression of CmCADs, and CmCAD5 is expressed in different vegetative tissues except mature leaves, with the highest expression in flower, and CmCAD5 is strongly expressed in flesh during development | Cucumis melo | - |
| additional information | differential expression of CmCADs, CmCAD2 is strongly expressed in flesh during development | Cucumis melo | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cinnamyl alcohol + NADP+ | - |
Cucumis melo | cinnamaldehyde + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39200, about, sequence calculation | Cucumis melo |
| ? | x * 38420, about, sequence calculation | Cucumis melo |
| ? | x * 38870, about, sequence calculation | Cucumis melo |
| ? | x * 39400, about, sequence calculation | Cucumis melo |
| ? | x * 39620, about, sequence calculation | Cucumis melo |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD | - |
Cucumis melo |
| CAD1 | - |
Cucumis melo |
| CAD2 | - |
Cucumis melo |
| CAD3 | - |
Cucumis melo |
| CAD4 | - |
Cucumis melo |
| CAD5 | - |
Cucumis melo |
| cinnamyl alcohol dehydrogenase | - |
Cucumis melo |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Cucumis melo | |
| NADPH | - |
Cucumis melo | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cucumis melo | sequence calculation | - |
5.68 |
| Cucumis melo | sequence calculation | - |
5.96 |
| Cucumis melo | sequence calculation | - |
5.99 |
| Cucumis melo | sequence calculation | - |
6.14 |
| Cucumis melo | sequence calculation | - |
6.63 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cucumis melo | ethylene induces the expression of CmCADs | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the phylogenetic tree reveals seven groups of CAD and melon CAD genes fall into four main groups. CmCAD1 and CmCAD2 belong to the bona fide CAD group, in which these CAD genes, as representative from angiosperms, are involved in lignin synthesis. Other CmCADs are distributed in group II, V and VII, respectively | Cucumis melo |
| metabolism | cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in lignin biosynthesis | Cucumis melo |
| physiological function | cinnamyl alcohol dehydrogenase (CAD) catalyses the final step of the lignin biosynthesis, the conversion of cinnamyl aldehydes to alcohols, using NADPH as a cofactor. CAD2 may be involved in the lignin biosynthesis induced by both abiotic and biotic stresses and in tissue-specific developmental lignification through a CAD genes family network, and CmCAD2 is the main CAD enzymes for lignification of melon flesh | Cucumis melo |
| physiological function | cinnamyl alcohol dehydrogenase (CAD) catalyses the final step of the lignin biosynthesis, the conversion of cinnamyl aldehydes to alcohols, using NADPH as a cofactor. CAD3 is involved in the lignin biosynthesis induced by both abiotic and biotic stresses and in tissue-specific developmental lignification through a CAD genes family network | Cucumis melo |
| physiological function | cinnamyl alcohol dehydrogenase (CAD) catalyses the final step of the lignin biosynthesis, the conversion of cinnamyl aldehydes to alcohols, using NADPH as a cofactor. CmCAD1 is involved in the lignin biosynthesis induced by both abiotic and biotic stresses and in tissue-specific developmental lignification through a CAD genes family network | Cucumis melo |
| physiological function | cinnamyl alcohol dehydrogenase (CAD) catalyses the final step of the lignin biosynthesis, the conversion of cinnamyl aldehydes to alcohols, using NADPH as a cofactor. CmCAD5 is involved in the lignin biosynthesis induced by both abiotic and biotic stresses and in tissue-specific developmental lignification through a CAD genes family network, CmCAD5 may also function in flower development | Cucumis melo |
| physiological function | CmCAD4 may be a pseudogene | Cucumis melo |
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